affinity chromatography technology

Go to external page http://purl.obolibrary.org/obo/MI_0004


This class of approaches is characterised by the use of affinity resins as tools to purify molecule of interest (baits) and their binding partners. The baits can be captured by a variety of high affinity ligands linked to a resin - for example, antibodies specific for the bait itself, antibodies for specific tags engineered to be expressed as part of the bait or other high affinity binders such as glutathione resins for GST fusion proteins, metal resins for histidine-tagged proteins. [ http://www.ncbi.nlm.nih.gov/pubmed/7708014 ]

This is just here as a test because I lose it

Term information

Subsets

PSI-MI_slim

definition

This class of approaches is characterised by the use of affinity resins as tools to purify molecule of interest (baits) and their binding partners. The baits can be captured by a variety of high affinity ligands linked to a resin - for example, antibodies specific for the bait itself, antibodies for specific tags engineered to be expressed as part of the bait or other high affinity binders such as glutathione resins for GST fusion proteins, metal resins for histidine-tagged proteins.

has exact synonym

Affinity purification

id

MI:0004

Term relations