This is a subclone of the mouse myoblast cell line. The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [ ATCC_American_Cell_Type_Culture_Collection:http://www.lgcstandards-atcc.org/ ]