{"data":{"type":"samples","id":"ERS739149","attributes":{"latitude":null,"longitude":null,"biosample":"SAMEA3403099","sample-metadata":[{"key":"ENA checklist","value":"ERC000011","unit":null}],"accession":"ERS739149","analysis-completed":null,"collection-date":null,"geo-loc-name":null,"sample-desc":"Protocols: Steady state mice not treated For simultaneous DNA and RNA extraction, pellets were dissolved in 500 ul extraction buffer (200 mM NaCl, 20 mM EDTA, 4 M guanidine thiocyanate, 1 % (v/v) β-mercaptoethanol) and 210 ul 20 % (w/v) SDS and lysed with a bead beater twice for 2 minutes employing Lysing Matrix E tubes (MP Biomedicals). Samples were centrifuged at 8000 g for 5 minutes and the supernatant was diluted with 3 volumes of lysing buffer (ZR-Duet DNA/RNA Miniprep Kit, Zymo Research). DNA and RNA were further extracted employing the ZR-Duet DNA/RNA Miniprep Kit according to the manufacturer’s instructions. Total RNA was depleted of ribosomal RNA using Epibio’s universal bacteria ribo-zero kit, single version. Libraries were constructed using the NEBNext Ultra directional RNA Library Prep Kit (E7420L).","environment-biome":null,"environment-feature":null,"environment-material":null,"sample-name":"E-MTAB-3562:rna_stool-ss-R2","sample-alias":"E-MTAB-3562:rna_stool-ss-R2","host-tax-id":null,"species":null,"last-update":"2023-03-04T00:59:53"},"relationships":{"biome":{"data":{"type":"biomes","id":"root:Host-associated:Mammals:Gastrointestinal tract:Intestine:Fecal"},"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Mammals:Gastrointestinal%20tract:Intestine:Fecal?format=json"}},"studies":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS739149/studies?format=json"},"data":[{"type":"studies","id":"MGYS00006101","links":{"self":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00006101?format=json"}}]},"runs":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS739149/runs?format=json"}}},"links":{"self":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS739149?format=json"}}}