{"data":{"type":"samples","id":"ERS167166","attributes":{"sample-metadata":[{"key":"sample storage temperature","value":"-80","unit":"°C"},{"key":"sex","value":"male","unit":null},{"key":"investigation type","value":"MIxS","unit":null},{"key":"project name","value":"Follow-up-IBS","unit":null},{"key":"geographic location (longitude)","value":"-0.3775","unit":null},{"key":"geographic location (country and/or sea,region)","value":"Spain","unit":null},{"key":"collection date","value":"2010-07-20","unit":null},{"key":"environment (biome)","value":"terrestrial biome ENVO:00000446","unit":null},{"key":"environment (feature)","value":"human-associated habitat","unit":null},{"key":"environment (material)","value":"feces","unit":null},{"key":"environmental package","value":"human-gut","unit":null},{"key":"sample collection device or method","value":"Feces were self-collected by the volunteers in tubes containing 10 mL of phosphate buffered saline (PBS). Samples were kept at 4 �C until their storage at -80 �C (approximately 2 hours after being collected)","unit":null},{"key":"sample material processing","value":"Before nucleic acid extraction, fecal samples were resuspended in PBS and centrifuged at 4,000 rpm for 3 min to remove fecal debris. 1-4 mL of the supernatants were centrifuged at 14,000 rpm for 3 min to recover cell pellets","unit":null},{"key":"nucleic acid extraction","value":"DNA and RNA were simultaneously extracted from cell pellets using the AllPrep DNA/RNA Mini Kit (QIAGEN)","unit":null},{"key":"sequencing method","value":"Pyrosequencing","unit":null},{"key":"age","value":"66","unit":null},{"key":"body product","value":"feces","unit":null},{"key":"body-mass index","value":"31.7","unit":null},{"key":"diet","value":"mediterranean diet","unit":null},{"key":"disease status","value":"not applicable","unit":null},{"key":"family relationship","value":"husband of P1","unit":null},{"key":"host common name","value":"Homo sapiens","unit":null},{"key":"host taxid","value":"9606","unit":null},{"key":"IHMC ethnicity","value":"caucasian","unit":null},{"key":"geographic location (latitude)","value":"39.469723","unit":null},{"key":"gastrointestinal tract disorder","value":"none","unit":null},{"key":"smoker","value":"ex-smoker","unit":null},{"key":"GOLD sample classification","value":"Host-associated > Human > Digestive system > Large intestine > Fecal","unit":null},{"key":"NCBI sample classification","value":"408170","unit":null},{"key":"instrument model","value":"454 GS FLX Titanium","unit":null},{"key":"ENA checklist","value":"GSC:MIxS v2.1","unit":null},{"key":"host sex","value":"male","unit":null},{"key":"host scientific name","value":"Homo sapiens","unit":null}],"latitude":39.4697,"biosample":"SAMEA2164648","longitude":-0.3775,"accession":"ERS167166","analysis-completed":"2012-08-29","collection-date":"2010-07-20","geo-loc-name":"Spain; Valencia","sample-desc":"fecal sample of Control 1 on day 28 of the follow-up","environment-biome":"terrestrial biome ENVO:00000446","environment-feature":"human-associated habitat","environment-material":"feces","sample-name":"C1-D28","sample-alias":"C1-D28","host-tax-id":9606,"species":"Homo sapiens","last-update":"2022-04-11T09:04:13"},"relationships":{"studies":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS167166/studies?format=json"},"data":[{"type":"studies","id":"MGYS00000308","links":{"self":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000308?format=json"}},{"type":"studies","id":"MGYS00006008","links":{"self":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00006008?format=json"}}]},"runs":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS167166/runs?format=json"}},"biome":{"data":{"type":"biomes","id":"root:Host-associated:Human:Digestive system:Large intestine:Fecal"},"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Human:Digestive%20system:Large%20intestine:Fecal?format=json"}}},"links":{"self":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS167166?format=json"}}}