{"links":{"first":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/DRS012355/studies?format=json&page=1","last":"https://www.ebi.ac.uk/metagenomics/api/v1/samples/DRS012355/studies?format=json&page=1","next":null,"prev":null},"data":[{"type":"studies","id":"MGYS00002476","attributes":{"samples-count":18,"bioproject":"PRJDB1919","accession":"MGYS00002476","is-private":false,"last-update":"2024-01-23T12:46:04","secondary-accession":"DRP001207","centre-name":"Fisheries Research Agency","public-release-date":null,"study-abstract":"In the present study, we performed a detailed investigation of the 18S-rDNA, namely, numbers of registered sequences, frequencies of amplification success, the amplicon sequence variability among three regions containing V1-V3, V4-V5 and V7-V9 regions using in silico PCRs based on public databases, and the identification power by NGS-based environmental surveys of planktonic eukaryote community. Although the number of registered sequences in V4-V5 regions was remarkably higher than other regions, the identification power in NGS-based environmental surveys was lowest in V4-V5 regions due to the lowest sequence variability. The number of registered sequences in V1-V3 region was ca. two times smaller than V7-V9 region, and the sequence variability in V1-V3 region was significantly higher than that in V7-V9 region. Then, the identification power was not significant between these two regions, implying identification power is affected by combination of numbers of registered sequences and the sequence variability. We therefore believe V1-V3 region will be the best one for applying to NGS-based monitoring of planktonic eukaryote community in the near future as the number of sequences deposited increases in public databases.","study-name":"Comparative study in validity of three regions of 18S-rRNA for eukaryote amplicon sequence analyses","data-origination":"HARVESTED"},"relationships":{"downloads":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00002476/downloads?format=json"}},"geocoordinates":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00002476/geocoordinates?format=json"}},"analyses":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00002476/analyses?format=json"}},"biomes":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00002476/biomes?format=json"},"data":[{"type":"biomes","id":"root:Environmental:Aquatic:Marine:Coastal","links":{"self":"https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Aquatic:Marine:Coastal?format=json"}}]},"samples":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00002476/samples?format=json"}},"publications":{"links":{"related":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00002476/publications?format=json"}}},"links":{"self":"https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00002476?format=json"}}],"meta":{"pagination":{"page":1,"pages":1,"count":1}}}