MTBLS774: Imidazole ketone erastin induces ferroptosis and slows tumor growth in a mouse lymphoma model

Abstract

Ferroptosis is a form of regulated cell death that can be induced by inhibition of the cystine-glutamate antiporter, system xc-. Among the existing system xc- inhibitors, imidazole ketone erastin (IKE) is a potent, metabolically stable inhibitor of system xc- and inducer of ferroptosis potentially suitable for in vivo applications. We investigated the pharmacokinetic and pharmacodynamic features of IKE in a diffuse large B cell lymphoma (DLBCL) xenograft model and demonstrated that IKE exerted an antitumor effect by inhibiting system xc-, leading to glutathione depletion, lipid peroxidation, and the induction of ferroptosis biomarkers both in vitro and in vivo. Using untargeted lipidomics and qPCR, we identified distinct features of lipid metabolism in IKE-induced ferroptosis. In addition, biodegradable polyethylene glycol-poly(lactic-co-glycolic acid) nanoparticles were employed to aid in IKE delivery and exhibited reduced toxicity compared with free IKE in a DLBCL xenograft model.

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 Authors: Brent Stockwell

  Release date: 17-Apr-2019

 Status: Public

Organism(s)

Homo sapiens/Mus Musculus Xenograft

Homo sapiens

Solvent Blank

Matrix

  Study Design

GO:ferroptosis

BAO:lymphoma

NCIT:Lipidomics

  Experimental Factors

replicate

Cell line

Animal model

Replicate

Drug 1

Concentration Drug 1

Drug 2

Concentration Drug 2

Timepoint

SCID model

Category

Protocol Description
Sample collection
In vitro study
SUDHL-6 cell line was obtained from ATCC and cultured in RPMI-1640 (ATCC 30-2001) with 10% FBS and 1% pen-strep, maintained in a humidified environment at 37 °C and 5% CO2 in an incubator. Cells were treated with DMSO, 1 μM IKE, or 500 nM IKE, with or without ferroptosis inhibitors 10 μM ferrostatin-1 (fer-1) and 10 μM β-mercaptoethanol (β-Me) or 10 μM deferoxamine (DFO) for 24 hrs.

In vivo study
The animal studies reported in this study adhered to the ARRIVE guidelines. All animal study protocols were approved by the Columbia University Institutional Animal Care and Use Committee (IACUC).
Male NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) (Charles River, strain code 572) mice 6 weeks of age were injected with 10 million SUDHL-6 cells subcutaneously. Visible tumors appeared after 2 weeks. Tumour size was measured by electronic caliper every 2 days and calculated using the formula: 0.5 x length x width2. After another 2 weeks, mice were randomly separated into group of 3 mice per cage with roughly tumor size (1,200 mm3). Mice were dosed at 50 mg/kg IKE by IP and samples were collected at 0, 1, 2, 3, 4, 6, and 24 h with three mice per time point. Control mice were dosed with DMSO vehicle by IP and samples were collected at 24 h. Mice were euthanized using a CO2 gas chamber before xenograft dissection. Tumors were dissected and divided into 6 segments, frozen on dry ice, and stored at -80 °C.
Extraction
In vitro study
The lipids were extracted from each cell sample using a modified Matyash method. Five million cells for each sample type were homogenized in 250 μl cold methanol containing 0.1% butylated hydroxyl toluene (BHT) with a micro tip sonicator. Homogenized samples were transferred to fresh glass tubes containing 850 μl of cold methyl-tert-butyl ether (MTBE) and vortex-mixed for 30 sec. To enhance extraction efficiency of lipids, the samples were incubated overnight at 4 °C on the shaker. On the next day, 200 μl of cold water was added to each sample, and incubated for 20 min on ice before centrifugation at 3,000 rpm for 20 min at 4 °C. The organic layer was collected followed by drying under a gentle stream of nitrogen gas on ice and stored at -80 °C until LC-MS analysis. The protein pellet was used to measure protein concentration using Bio-Rad protein assay. The samples were re-constituted in a solution containing IPA/ ACN /water (4:3:1, v/v/v) containing mixture of internal standard for further MS analysis.

In vivo study 
Frozen tissue (25 mg) was homogenized in 15 μl/mg pre-chilled methanol containing 0.1% butylated hydroxyl-toluene (BHT) at speed 5 for 30 secs using a Bead Ruptor 4 (OMNI International). Then, 300 μl of tissue lysate were transferred to a glass vials containing 1000 μl ice-cold methyl-tert-butyl ether (MTBE) and vortex-mixed for 30 sec. The sample are stored in the -20 °C freezer overnight to enhance lipids extraction. The following day, 250 μl of ice-cold methanol was added to each sample and vortex-mixed for 30 sec vigorously. Samples were incubated on dry ice for 20 min on the shaker followed by centrifugation at 3,000 rpm for 20 min at 4°C. Finally, 1000 μl of upper phase containing lipids were transferred to fresh glass vials, and evaporated to dryness under the stream of N2 gas. The samples were re-constituted in a solution containing IPA/ ACN /water (4:3:1, v/v/v) containing mixture of internal standard for further MS analysis.

Experimental controls
Blank; acetonitrile:water (50:50 v/v)
Matrix; 2-propanol:acetonitrile:water (4:3:1, v/v/v)
Internal standard; PLASH® LIPIDOMIX® Mass Spec Standard ((Avanti Polar Lipids, INC., Alabaster, AL, USA)
Quality control; a quality control (QC) sample was prepared by combining 40 μl of each sample to assess the reproducibility of the features through the runs.
Chromatography Chromatographic separation of extracted lipids was carried out at 55 °C on Acquity UPLC HSS T3 column (2.1× 100 mm, 1.8 μm) over a 17 min gradient elution. Mobile phase A consisted of ACN/water (60:40, v/v) and mobile phase B was IPA/ACN/water (85:10:5, v/v/v) both containing 10 mM ammonium acetate and 0.1% acetic acid. After injection, the gradient was held at 60% mobile phase A for 1.5 min. For the next 12 min, the gradient was ramped in a linear fashion to 100% B and held at this composition for 3 min. The eluent composition returned to the initial condition in 1 min, and the column was re-equilibrated for an additional 1 min before the next injection was conducted. The flow rate was set to 400 µl/min and injection volumes were 5 µl using the flow-through needle mode in both positive and negative ionization modes. The QC sample was injected between the samples and at the end of the run to monitor the performance and the stability of the MS platform. This QC sample was also injected at least 5 times at the beginning of the UPLC/MS run, in order to condition the column.
Mass spectrometry The Synapt G2 mass spectrometer (Waters, Manchester, U.K.) was operated in both positive and negative electrospray ionization (ESI) modes. For positive mode, a capillary voltage and sampling cone voltage of 3 kV and 32 V were used. The source and desolvation temperature were kept at 120 °C and 500 °C, respectively. Nitrogen was used as desolvation gas with a flow rate of 900 L/hr. For negative mode, a capillary voltage of -2 kV and a cone voltage of 30 V were used. The source temperature was 120 °C, and desolvation gas flow was set to 900 L/hr. Dependent on the ionization mode the protonated molecular ion of leucine encephalin ([M+H]+, m/z 556.2771) or the deprotonated molecular ion ( [M-H]-, m/z 554.2615) was used as a lock mass for mass accuracy and reproducibility. Leucine enkephalin was introduced to the lock mass at a concentration of 2 ng/µl (50% ACN containing 0.1% formic acid), and a flow rate of 10 µl/min. The data was collected in duplicates in the centroid data independent (MSE) mode over the mass range m/z 50 to 1600 Da with an acquisition time of 0.1 secs per scan. The QC samples were also acquired in enhanced data independent ion mobility (IMS-MSE) in both positive and negative modes for enhancing the structural assignment of lipid species. The ESI source settings were the same as described above. The traveling wave velocity was set to 650 m/s and wave height was 40 V. The helium gas flow in the helium cell region of the ion-mobility spectrometry (IMS) cell was set to 180 ml/min to reduce the internal energy of the ions and minimize fragmentation. Nitrogen as the drift gas was held at a flow rate of 90 ml/min in the IMS cell. The low collision energy was set to 4 eV, and high collision energy was ramping from 25 to 65 eV in the transfer region of the T-Wave device to induce fragmentation of mobility-separated precursor ions.
Data transformation
All raw data files were converted to netCDF format using DataBridge tool implemented in MassLynx software (Waters, version 4.1). Then, they were subjected to peak-picking, retention time alignment, and grouping using XCMS package6 (version 3.2.0) in R (version 3.5.1) environment. For the peak picking, the CentWave algorithm7 was used with the peak width window of 2-25 s. For peak grouping, bandwidth and m/z-width of 2 s and 0.01 Da were used, respectively. After retention time alignment and filling missing peaks, an output data frame was generated containing the list of time-aligned detected features (m/z and retention time) and the relative signal intensity (area of the chromatographic peak) in each sample. Technical variations such as noise were assessed and removed from extracted features' list based on the ratios of average relative signal intensities of the blanks to QC samples (blank/QC >1.5). Also, peaks with variations larger than 30% in QCs were eliminated. Multivariate and univariate analyses were performed using MetaboAnalyst 8(version 4.0) and in R (version 3.5.1) environment. Group differences were calculated using VIP scores of PLS-DA model and one-way ANOVA (p < 0.05) and false discovery rate of 5% to control for multiple comparisons.
Metabolite identification Structural elucidation and validation of significant features were initially obtained by searching monoisotopic masses against the available online databases such as METLIN, HMDB, LipidMAPS, with a mass tolerance of 5 ppm. Fragment ion information obtained by tandem MS (UPLC-IMS-MSE) analysis for further structural elucidation. IMS-MSE data were processed using MSE viewer (Version 1.3, Waters Corp., MA, USA).
Source Name Organism Organism part Cell line Animal model Protocol REF Sample Name replicate Category Drug 1 Drug 1 concentration Unit Drug 2 Drug 2 Unit Timepoint Unit
SUDHL6-Cells-Positive-sample1 Solvent Blank Mixture Sample collection SEP29.18.BLANK.POS.201 Exp1-Sample-1 Blank micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample2 Matrix Mixture Sample collection SEP29.18.MATRIX.POS.101 Exp1-Sample-2 Experimental control micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample3 Matrix Mixture Sample collection SEP29.18.MATRIX.POS.1A01 Exp1-Sample-3 Experimental control micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample4 Matrix Mixture Sample collection SEP29.18.MATRIX.POS.201 Exp1-Sample-4 Experimental control micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample5 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.1A.POS01 Exp1-Sample-5 Drug vehicle control DMSO micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample6 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.1B.POS01 Exp1-Sample-6 Drug vehicle control DMSO micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample7 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.2A.POS01 Exp1-Sample-7 Drug vehicle control DMSO micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample8 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.2B.POS01 Exp1-Sample-8 Drug vehicle control DMSO micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample9 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.3A.POS01 Exp1-Sample-9 Drug vehicle control DMSO micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample10 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.3B.POS01 Exp1-Sample-10 Drug vehicle control DMSO micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample11 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.1A.POS01 Exp1-Sample-11 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample12 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.1B.POS01 Exp1-Sample-12 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample13 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.2A.POS01 Exp1-Sample-13 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample14 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.2B.POS01 Exp1-Sample-14 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample15 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.3A.POS01 Exp1-Sample-15 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample16 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.3B.POS01 Exp1-Sample-16 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample17 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.1A.POS01 Exp1-Sample-17 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample18 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.1B.POS01 Exp1-Sample-18 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample19 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.2A.POS01 Exp1-Sample-19 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample20 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.2B.POS01 Exp1-Sample-20 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample21 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.3A.POS01 Exp1-Sample-21 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample22 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.3B.POS01 Exp1-Sample-22 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Positive-sample23 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.1A.POS01 Exp1-Sample-23 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample24 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.1B.POS01 Exp1-Sample-24 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample25 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.2A.POS01 Exp1-Sample-25 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample26 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.2B.POS01 Exp1-Sample-26 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample27 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.3A.POS01 Exp1-Sample-27 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample28 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.3B.POS01 Exp1-Sample-28 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample29 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.1A.POS01 Exp1-Sample-29 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample30 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.1B.POS01 Exp1-Sample-30 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample31 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.2A.POS01 Exp1-Sample-31 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample32 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.2B.POS01 Exp1-Sample-32 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample33 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.3A.POS01 Exp1-Sample-33 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample34 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.3B.POS01 Exp1-Sample-34 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample35 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.1A.POS01 Exp1-Sample-35 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample36 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.1B.POS01 Exp1-Sample-36 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample37 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.2A.POS01 Exp1-Sample-37 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample38 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.2B.POS01 Exp1-Sample-38 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample39 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.3A.POS01 Exp1-Sample-39 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample40 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.3B.POS01 Exp1-Sample-40 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Positive-sample41 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.POS.101 Exp1-Sample-41 Pooled sample 24 Hour
SUDHL6-Cells-Positive-sample42 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.POS.201 Exp1-Sample-42 Pooled sample 24 Hour
SUDHL6-Cells-Positive-sample43 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.POS.301 Exp1-Sample-43 Pooled sample 24 Hour
SUDHL6-Cells-Positive-sample44 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.POS.401 Exp1-Sample-44 Pooled sample 24 Hour
SUDHL6-Cells-Positive-sample45 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.POS.501 Exp1-Sample-45 Pooled sample 24 Hour
SUDHL6-Cells-Positive-sample46 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.POS.601 Exp1-Sample-46 Pooled sample 24 Hour
SUDHL6-Cells-Positive-sample47 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.POS.701 Exp1-Sample-47 Pooled sample 24 Hour
SUDHL6-Cells-Negative-sample1 Solvent Blank Mixture Sample collection SEP27.18.BLANK.NEG.101 Exp1-Sample-1 Blank 24 Hour
SUDHL6-Cells-Negative-sample2 Matrix Mixture Sample collection SEP27.18.BLANK.NEG.201 Exp1-Sample-2 Experimental control 24 Hour
SUDHL6-Cells-Negative-sample3 Matrix Mixture Sample collection SEP27.18.MATRIX.NEG.101 Exp1-Sample-3 Experimental control 24 Hour
SUDHL6-Cells-Negative-sample4 Matrix Mixture Sample collection SEP27.18.MATRIX.NEG.201 Exp1-Sample-4 Experimental control 24 Hour
SUDHL6-Cells-Negative-sample5 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.1A01 Exp1-Sample-5 Drug vehicle control DMSO 24 Hour
SUDHL6-Cells-Negative-sample6 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.1B01 Exp1-Sample-6 Drug vehicle control DMSO 24 Hour
SUDHL6-Cells-Negative-sample7 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.2A01 Exp1-Sample-7 Drug vehicle control DMSO 24 Hour
SUDHL6-Cells-Negative-sample8 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.2B01 Exp1-Sample-8 Drug vehicle control DMSO 24 Hour
SUDHL6-Cells-Negative-sample9 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.3A01 Exp1-Sample-9 Drug vehicle control DMSO 24 Hour
SUDHL6-Cells-Negative-sample10 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection DMSO.3B01 Exp1-Sample-10 Drug vehicle control DMSO 24 Hour
SUDHL6-Cells-Negative-sample11 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.1A01 Exp1-Sample-11 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample12 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.1B01 Exp1-Sample-12 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample13 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.2A01 Exp1-Sample-13 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample14 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.2B01 Exp1-Sample-14 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample15 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.3A01 Exp1-Sample-15 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample16 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.1UM.3B01 Exp1-Sample-16 Treated imidazole ketone erastin 1 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample17 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.1A01 Exp1-Sample-17 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample18 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.1B01 Exp1-Sample-18 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample19 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.2A01 Exp1-Sample-19 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample20 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.2B01 Exp1-Sample-20 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample21 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.3A01 Exp1-Sample-21 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample22 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE.500NM.3B01 Exp1-Sample-22 Treated imidazole ketone erastin 0.5 micromolar micromolar 24 Hour
SUDHL6-Cells-Negative-sample23 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.1A01 Exp1-Sample-23 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample24 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.1B01 Exp1-Sample-24 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample25 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.2A01 Exp1-Sample-25 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample26 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.2B01 Exp1-Sample-26 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample27 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.3A01 Exp1-Sample-27 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample28 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_BME.3B01 Exp1-Sample-28 Treated imidazole ketone erastin 0.5 micromolar Beta-mercaptoethanol 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample29 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.1A01 Exp1-Sample-29 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample30 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.1B01 Exp1-Sample-30 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample31 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.2A01 Exp1-Sample-31 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample32 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.2B01 Exp1-Sample-32 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample33 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.3A01 Exp1-Sample-33 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample34 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_DFO.3B01 Exp1-Sample-34 Treated imidazole ketone erastin 0.5 micromolar deferoxamine 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample35 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.1A01 Exp1-Sample-35 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample36 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.1B01 Exp1-Sample-36 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample37 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.2A01 Exp1-Sample-37 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample38 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.2B01 Exp1-Sample-38 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample39 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.3A01 Exp1-Sample-39 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample40 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection IKE_FER.3B01 Exp1-Sample-40 Treated imidazole ketone erastin 0.5 micromolar ferrostatin-1 10 micromolar 24 Hour
SUDHL6-Cells-Negative-sample41 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.NEG.101 Exp1-Sample-41 Pooled sample 24 Hour
SUDHL6-Cells-Negative-sample42 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.NEG.201 Exp1-Sample-42 Pooled sample 24 Hour
SUDHL6-Cells-Negative-sample43 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.NEG.301 Exp1-Sample-43 Pooled sample 24 Hour
SUDHL6-Cells-Negative-sample44 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.NEG.401 Exp1-Sample-44 Pooled sample 24 Hour
SUDHL6-Cells-Negative-sample45 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.NEG.501 Exp1-Sample-45 Pooled sample 24 Hour
SUDHL6-Cells-Negative-sample46 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.NEG.601 Exp1-Sample-46 Pooled sample 24 Hour
SUDHL6-Cells-Negative-sample47 Homo sapiens Diffuse Large B Cell Lymphoma SU-DHL-6 Sample collection SEP27.18.QC.NEG.701 Exp1-Sample-47 Pooled sample 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-1 Solvent Blank Mixture Sample collection AUG31.BLANK.101 Exp2-Sample-1 Blank Hour
Mouse-Xenograft-Positive-Tumor tissue-2 Solvent Blank Mixture Sample collection AUG31.BLANK.201 Exp2-Sample-2 Blank Hour
Mouse-Xenograft-Positive-Tumor tissue-3 Matrix Mixture Sample collection AUG31.MATRIX.101 Exp2-Sample-3 Experimental control Hour
Mouse-Xenograft-Positive-Tumor tissue-4 Matrix Mixture Sample collection AUG31.MATRIX.201 Exp2-Sample-4 Experimental control Hour
Mouse-Xenograft-Positive-Tumor tissue-5 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.101 Exp2-Sample-5 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-6 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.201 Exp2-Sample-6 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-7 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.301 Exp2-Sample-7 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-8 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.401 Exp2-Sample-8 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-9 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.501 Exp2-Sample-9 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-10 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.601 Exp2-Sample-10 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-11 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.701 Exp2-Sample-11 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-12 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31.QC.POS.801 Exp2-Sample-12 Pooled sample Hour
Mouse-Xenograft-Positive-Tumor tissue-13 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_DMSO.1A01 Exp2-Sample-13 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-14 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_DMSO.1B01 Exp2-Sample-14 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-15 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_DMSO.2A01 Exp2-Sample-15 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-16 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_DMSO.2B01 Exp2-Sample-16 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-17 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_DMSO.3A01 Exp2-Sample-17 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-18 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_DMSO.3B01 Exp2-Sample-18 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-19 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_0H.1A01 Exp2-Sample-19 Non treated 0 Hour
Mouse-Xenograft-Positive-Tumor tissue-20 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_0H.1B01 Exp2-Sample-20 Non treated 0 Hour
Mouse-Xenograft-Positive-Tumor tissue-21 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_0H.2A01 Exp2-Sample-21 Non treated 0 Hour
Mouse-Xenograft-Positive-Tumor tissue-22 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_0H.2B01 Exp2-Sample-22 Non treated 0 Hour
Mouse-Xenograft-Positive-Tumor tissue-23 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_0H.3A01 Exp2-Sample-23 Non treated 0 Hour
Mouse-Xenograft-Positive-Tumor tissue-24 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_0H.3B01 Exp2-Sample-24 Non treated 0 Hour
Mouse-Xenograft-Positive-Tumor tissue-25 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_1H.1A01 Exp2-Sample-25 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Positive-Tumor tissue-26 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_1H.1B01 Exp2-Sample-26 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Positive-Tumor tissue-27 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_1H.2A01 Exp2-Sample-27 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Positive-Tumor tissue-28 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_1H.2B01 Exp2-Sample-28 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Positive-Tumor tissue-29 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_1H.3A01 Exp2-Sample-29 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Positive-Tumor tissue-30 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_1H.3B01 Exp2-Sample-30 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Positive-Tumor tissue-31 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_2H.1A01 Exp2-Sample-31 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Positive-Tumor tissue-32 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_2H.1B01 Exp2-Sample-32 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Positive-Tumor tissue-33 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_2H.2A01 Exp2-Sample-33 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Positive-Tumor tissue-34 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_2H.2B01 Exp2-Sample-34 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Positive-Tumor tissue-35 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_2H.3A01 Exp2-Sample-35 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Positive-Tumor tissue-36 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_2H.3B01 Exp2-Sample-36 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Positive-Tumor tissue-37 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_3H.1A01 Exp2-Sample-37 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Positive-Tumor tissue-38 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_3H.1B01 Exp2-Sample-38 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Positive-Tumor tissue-39 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_3H.2A01 Exp2-Sample-39 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Positive-Tumor tissue-40 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_3H.2B01 Exp2-Sample-40 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Positive-Tumor tissue-41 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_3H.3A01 Exp2-Sample-41 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Positive-Tumor tissue-42 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_3H.3B01 Exp2-Sample-42 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Positive-Tumor tissue-43 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_4H.1A01 Exp2-Sample-43 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Positive-Tumor tissue-44 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_4H.1B01 Exp2-Sample-44 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Positive-Tumor tissue-45 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_4H.2A01 Exp2-Sample-45 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Positive-Tumor tissue-46 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_4H.2B01 Exp2-Sample-46 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Positive-Tumor tissue-47 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_4H.3A01 Exp2-Sample-47 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Positive-Tumor tissue-48 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_4H.3B01 Exp2-Sample-48 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Positive-Tumor tissue-49 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_6H.1A01 Exp2-Sample-49 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Positive-Tumor tissue-50 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_6H.1B01 Exp2-Sample-50 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Positive-Tumor tissue-51 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_6H.2A01 Exp2-Sample-51 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Positive-Tumor tissue-52 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_6H.2B01 Exp2-Sample-52 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Positive-Tumor tissue-53 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_6H.3A01 Exp2-Sample-53 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Positive-Tumor tissue-54 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_6H.3B01 Exp2-Sample-54 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Positive-Tumor tissue-55 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_24H.1A01 Exp2-Sample-55 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-56 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_24H.1B01 Exp2-Sample-56 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-57 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_24H.2A01 Exp2-Sample-57 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-58 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_24H.2B01 Exp2-Sample-58 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-59 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_24H.3A01 Exp2-Sample-59 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Positive-Tumor tissue-60 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG31_IKE_24H.3B01 Exp2-Sample-60 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-1 Solvent Blank Mixture Sample collection AUG29.BLANK.101 Exp2-Sample-1 Blank Hour
Mouse-Xenograft-Negative-Tumor tissue-2 Solvent Blank Mixture Sample collection AUG29.BLANK.201 Exp2-Sample-2 Blank Hour
Mouse-Xenograft-Negative-Tumor tissue-3 Matrix Mixture Sample collection AUG29.MATRIX.101 Exp2-Sample-3 Experimental control Hour
Mouse-Xenograft-Negative-Tumor tissue-4 Matrix Mixture Sample collection AUG29.MATRIX.1A01 Exp2-Sample-4 Experimental control Hour
Mouse-Xenograft-Negative-Tumor tissue-5 Matrix Mixture Sample collection AUG29.MATRIX.201 Exp2-Sample-5 Experimental control Hour
Mouse-Xenograft-Negative-Tumor tissue-6 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.101 Exp2-Sample-6 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-7 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.201 Exp2-Sample-7 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-8 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.301 Exp2-Sample-8 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-9 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.401 Exp2-Sample-9 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-10 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.501 Exp2-Sample-10 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-11 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.601 Exp2-Sample-11 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-12 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.701 Exp2-Sample-12 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-13 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29.QC.NEG.801 Exp2-Sample-13 Pooled sample Hour
Mouse-Xenograft-Negative-Tumor tissue-14 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_24H.1A01 Exp2-Sample-14 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-15 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_24H.1B01 Exp2-Sample-15 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-16 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_24H.2A01 Exp2-Sample-16 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-17 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_24H.2B01 Exp2-Sample-17 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-18 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_24H.3A01 Exp2-Sample-18 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-19 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_24H.3B01 Exp2-Sample-19 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-20 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_4H.1A01 Exp2-Sample-20 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Negative-Tumor tissue-21 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_4H.1B01 Exp2-Sample-21 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Negative-Tumor tissue-22 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_4H.2A01 Exp2-Sample-22 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Negative-Tumor tissue-23 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_4H.2B01 Exp2-Sample-23 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Negative-Tumor tissue-24 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_4H.3A01 Exp2-Sample-24 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Negative-Tumor tissue-25 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_4H.3B01 Exp2-Sample-25 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 4 Hour
Mouse-Xenograft-Negative-Tumor tissue-26 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_6H.1A01 Exp2-Sample-26 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Negative-Tumor tissue-27 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_6H.1B01 Exp2-Sample-27 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Negative-Tumor tissue-28 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_6H.2A01 Exp2-Sample-28 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Negative-Tumor tissue-29 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_6H.2B01 Exp2-Sample-29 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Negative-Tumor tissue-30 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_6H.3A01 Exp2-Sample-30 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Negative-Tumor tissue-31 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_6H.3B01 Exp2-Sample-31 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 6 Hour
Mouse-Xenograft-Negative-Tumor tissue-32 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_DMSO.1A01 Exp2-Sample-32 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-33 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_DMSO.1B01 Exp2-Sample-33 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-34 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_DMSO.2A01 Exp2-Sample-34 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-35 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_DMSO.2B01 Exp2-Sample-35 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-36 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_DMSO.3A01 Exp2-Sample-36 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-37 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_DMSO.3B01 Exp2-Sample-37 Drug vehicle control DMSO 24 Hour
Mouse-Xenograft-Negative-Tumor tissue-38 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_0H.1A01 Exp2-Sample-38 Non treated 0 Hour
Mouse-Xenograft-Negative-Tumor tissue-39 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_0H.1B01 Exp2-Sample-39 Non treated 0 Hour
Mouse-Xenograft-Negative-Tumor tissue-40 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_0H.2A01 Exp2-Sample-40 Non treated 0 Hour
Mouse-Xenograft-Negative-Tumor tissue-41 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_0H.2B01 Exp2-Sample-41 Non treated 0 Hour
Mouse-Xenograft-Negative-Tumor tissue-42 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_0H.3A01 Exp2-Sample-42 Non treated 0 Hour
Mouse-Xenograft-Negative-Tumor tissue-43 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_0H.3B01 Exp2-Sample-43 Non treated 0 Hour
Mouse-Xenograft-Negative-Tumor tissue-44 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_1H.1A01 Exp2-Sample-44 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Negative-Tumor tissue-45 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_1H.1B01 Exp2-Sample-45 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Negative-Tumor tissue-46 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_1H.2A01 Exp2-Sample-46 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Negative-Tumor tissue-47 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_1H.2B01 Exp2-Sample-47 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Negative-Tumor tissue-48 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_1H.3A01 Exp2-Sample-48 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Negative-Tumor tissue-49 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_1H.3B01 Exp2-Sample-49 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 1 Hour
Mouse-Xenograft-Negative-Tumor tissue-50 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_2H.1A01 Exp2-Sample-50 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Negative-Tumor tissue-51 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_2H.1B01 Exp2-Sample-51 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Negative-Tumor tissue-52 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_2H.2A01 Exp2-Sample-52 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Negative-Tumor tissue-53 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_2H.2B01 Exp2-Sample-53 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Negative-Tumor tissue-54 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_2H.3A01 Exp2-Sample-54 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Negative-Tumor tissue-55 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_2H.3B01 Exp2-Sample-55 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 2 Hour
Mouse-Xenograft-Negative-Tumor tissue-56 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_3H.1A01 Exp2-Sample-56 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Negative-Tumor tissue-57 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_3H.1B01 Exp2-Sample-57 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Negative-Tumor tissue-58 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_3H.2A01 Exp2-Sample-58 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Negative-Tumor tissue-59 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_3H.2B01 Exp2-Sample-59 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Negative-Tumor tissue-60 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_3H.3A01 Exp2-Sample-60 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour
Mouse-Xenograft-Negative-Tumor tissue-61 Homo sapiens/Mus Musculus Xenograft Diffuse Large B Cell Lymphoma SU-DHL-6 NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice (Charles River, strain code 572) Sample collection AUG29_IKE_3H.3B01 Exp2-Sample-61 Treated imidazole ketone erastin 50 Milligram Per Kilogram Body Weight 3 Hour

Assay  1

Assay file name: a_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
Technology: mass spectrometry
Platform: Liquid Chromatography MS - Negative (LC-MS (Negative))

Instrumentation

Sample Name Protocol REF Post Extraction Derivatization Extract Name Protocol REF Chromatography Instrument Column model Column type Labeled Extract Name Label Protocol REF Scan polarity Scan m/z range Instrument Ion source Mass analyzer MS Assay Name Raw Spectral Data File Protocol REF Normalization Name Derived Spectral Data File Protocol REF Data Transformation Name Metabolite Assignment File
SEP27.18.BLANK.NEG.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.BLANK.NEG.101 SEP27-18-QC-NEG-101.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.BLANK.NEG.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.BLANK.NEG.201 SEP27-18-QC-NEG-201.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.MATRIX.NEG.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.MATRIX.NEG.101 SEP27-18-MATRIX-NEG-101.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.MATRIX.NEG.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.MATRIX.NEG.201 SEP27-18-MATRIX-NEG-201.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.1A01 DMSO-1A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.1B01 DMSO-1B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.2A01 DMSO-2A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.2B01 DMSO-2B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.3A01 DMSO-3A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.3B01 DMSO-3B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.1A01 IKE-1UM-1A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.1B01 IKE-1UM-1B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.2A01 IKE-1UM-2A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.2B01 IKE-1UM-2B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.3A01 IKE-1UM-3A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.3B01 IKE-1UM-3B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.1A01 IKE-500NM-1A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.1B01 IKE-500NM-1B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.2A01 IKE-500NM-2A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.2B01 IKE-500NM-2B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.3A01 IKE-500NM-3A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.3B01 IKE-500NM-3B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.1A01 IKE_BME-1A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.1B01 IKE_BME-1B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.2A01 IKE_BME-2A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.2B01 IKE_BME-2B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.3A01 IKE_BME-3A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.3B01 IKE_BME-3B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.1A01 IKE_DFO-1A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.1B01 IKE_DFO-1B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.2A01 IKE_DFO-2A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.2B01 IKE_DFO-2B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.3A01 IKE_DFO-3A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.3B01 IKE_DFO-3B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.1A01 IKE_FER-1A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.1B01 IKE_FER-1B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.2A01 IKE_FER-2A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.2B01 IKE_FER-2B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.3A01 IKE_FER-3A01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.3B01 IKE_FER-3B01.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.NEG.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.NEG.101 SEP27-18-QC-NEG-101.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.NEG.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.NEG.201 SEP27-18-QC-NEG-201.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.NEG.301 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.NEG.301 SEP27-18-QC-NEG-301.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.NEG.401 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.NEG.401 SEP27-18-QC-NEG-401.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.NEG.501 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.NEG.501 SEP27-18-QC-NEG-501.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.NEG.601 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.NEG.601 SEP27-18-QC-NEG-601.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.NEG.701 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.NEG.701 SEP27-18-QC-NEG-701.CDF Data transformation Metabolite identification m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv

Assay  2

Assay file name: a_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
Technology: mass spectrometry
Platform: Liquid Chromatography MS - Positive (LC-MS (Positive))

Instrumentation

Sample Name Protocol REF Post Extraction Derivatization Extract Name Protocol REF Chromatography Instrument Column model Column type Labeled Extract Name Label Protocol REF Scan polarity Scan m/z range Instrument Ion source Mass analyzer MS Assay Name Raw Spectral Data File Protocol REF Normalization Name Derived Spectral Data File Protocol REF Data Transformation Name Metabolite Assignment File
SEP29.18.BLANK.POS.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP29.18.BLANK.POS.201 SEP29-18-BLANK-POS-201.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP29.18.MATRIX.POS.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP29.18.MATRIX.POS.101 SEP29-18-MATRIX-POS-101.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP29.18.MATRIX.POS.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP29.18.MATRIX.POS.1A01 SEP29-18-MATRIX-POS-1A01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP29.18.MATRIX.POS.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP29.18.MATRIX.POS.201 SEP29-18-MATRIX-POS-201.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.1A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.1A.POS01 DMSO-1A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.1B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.1B.POS01 DMSO-1B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.2A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.2A.POS01 DMSO-2A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.2B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.2B.POS01 DMSO-2B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.3A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.3A.POS01 DMSO-3A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
DMSO.3B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight DMSO.3B.POS01 DMSO-3B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.1A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.1A.POS01 IKE-1UM-1A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.1B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.1B.POS01 IKE-1UM-1B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.2A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.2A.POS01 IKE-1UM-2A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.2B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.2B.POS01 IKE-1UM-2B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.3A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.3A.POS01 IKE-1UM-3A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.1UM.3B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.1UM.3B.POS01 IKE-1UM-3B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.1A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.1A.POS01 IKE-500NM-1A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.1B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.1B.POS01 IKE-500NM-1B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.2A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.2A.POS01 IKE-500NM-2A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.2B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.2B.POS01 IKE-500NM-2B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.3A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.3A.POS01 IKE-500NM-3A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE.500NM.3B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE.500NM.3B.POS01 IKE-500NM-3B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.1A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.1A.POS01 IKE_BME-1A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.1B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.1B.POS01 IKE_BME-1B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.2A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.2A.POS01 IKE_BME-2A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.2B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.2B.POS01 IKE_BME-2B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.3A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.3A.POS01 IKE_BME-3A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_BME.3B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_BME.3B.POS01 IKE_BME-3B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.1A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.1A.POS01 IKE_DFO-1A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.1B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.1B.POS01 IKE_DFO-1B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.2A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.2A.POS01 IKE_DFO-2A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.2B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.2B.POS01 IKE_DFO-2B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.3A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.3A.POS01 IKE_DFO-3A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_DFO.3B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_DFO.3B.POS01 IKE_DFO-3B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.1A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.1A.POS01 IKE_FER-1A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.1B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.1B.POS01 IKE_FER-1B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.2A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.2A.POS01 IKE_FER-2A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.2B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.2B.POS01 IKE_FER-2B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.3A.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.3A.POS01 IKE_FER-3A-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
IKE_FER.3B.POS01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight IKE_FER.3B.POS01 IKE_FER-3B-POS01.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.POS.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.POS.101 SEP27-18-QC-POS-101.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.POS.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.POS.201 SEP27-18-QC-POS-201.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.POS.301 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.POS.301 SEP27-18-QC-POS-301.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.POS.401 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.POS.401 SEP27-18-QC-POS-401.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.POS.501 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.POS.501 SEP27-18-QC-POS-501.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.POS.601 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.POS.601 SEP27-18-QC-POS-601.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
SEP27.18.QC.POS.701 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight SEP27.18.QC.POS.701 SEP27-18-QC-POS-701.CDF Data transformation Metabolite identification m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv

Assay  3

Assay file name: a_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
Technology: mass spectrometry
Platform: Liquid Chromatography MS - Negative (LC-MS (Negative))

Instrumentation

Sample Name Protocol REF Post Extraction Derivatization Extract Name Protocol REF Chromatography Instrument Column model Column type Labeled Extract Name Label Protocol REF Scan polarity Scan m/z range Instrument Ion source Mass analyzer MS Assay Name Raw Spectral Data File Protocol REF Normalization Name Derived Spectral Data File Protocol REF Data Transformation Name Metabolite Assignment File
AUG29.BLANK.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.BLANK.101 AUG29-BLANK-101.CDF Data transformation Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.BLANK.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.BLANK.201 AUG29-BLANK-201.CDF Data transformation Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.MATRIX.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.MATRIX.101 AUG29-MATRIX-101.CDF Data transformation Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.MATRIX.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.MATRIX.1A01 AUG29-MATRIX-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.MATRIX.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.MATRIX.201 AUG29-MATRIX-201.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.101 AUG29-QC-NEG-101.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.201 AUG29-QC-NEG-201.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.301 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.301 AUG29-QC-NEG-301.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.401 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.401 AUG29-QC-NEG-401.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.501 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.501 AUG29-QC-NEG-501.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.601 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.601 AUG29-QC-NEG-601.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.701 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.701 AUG29-QC-NEG-701.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29.QC.NEG.801 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29.QC.NEG.801 AUG29-QC-NEG-801.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_24H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_24H.1A01 AUG29_IKE_24H-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_24H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_24H.1B01 AUG29_IKE_24H-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_24H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_24H.2A01 AUG29_IKE_24H-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_24H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_24H.2B01 AUG29_IKE_24H-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_24H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_24H.3A01 AUG29_IKE_24H-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_24H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_24H.3B01 AUG29_IKE_24H-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_4H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_4H.1A01 AUG29_IKE_4H-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_4H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_4H.1B01 AUG29_IKE_4H-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_4H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_4H.2A01 AUG29_IKE_4H-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_4H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_4H.2B01 AUG29_IKE_4H-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_4H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_4H.3A01 AUG29_IKE_4H-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_4H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_4H.3B01 AUG29_IKE_4H-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_6H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_6H.1A01 AUG29_IKE_6H-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_6H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_6H.1B01 AUG29_IKE_6H-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_6H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_6H.2A01 AUG29_IKE_6H-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_6H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_6H.2B01 AUG29_IKE_6H-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_6H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_6H.3A01 AUG29_IKE_6H-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_6H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_6H.3B01 AUG29_IKE_6H-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_DMSO.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_DMSO.1A01 AUG29_DMSO-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_DMSO.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_DMSO.1B01 AUG29_DMSO-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_DMSO.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_DMSO.2A01 AUG29_DMSO-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_DMSO.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_DMSO.2B01 AUG29_DMSO-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_DMSO.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_DMSO.3A01 AUG29_DMSO-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_DMSO.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_DMSO.3B01 AUG29_DMSO-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_0H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_0H.1A01 AUG29_IKE_0H-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_0H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_0H.1B01 AUG29_IKE_0H-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_0H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_0H.2A01 AUG29_IKE_0H-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_0H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_0H.2B01 AUG29_IKE_0H-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_0H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_0H.3A01 AUG29_IKE_0H-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_0H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_0H.3B01 AUG29_IKE_0H-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_1H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_1H.1A01 AUG29_IKE_1H-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_1H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_1H.1B01 AUG29_IKE_1H-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_1H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_1H.2A01 AUG29_IKE_1H-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_1H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_1H.2B01 AUG29_IKE_1H-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_1H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_1H.3A01 AUG29_IKE_1H-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_1H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_1H.3B01 AUG29_IKE_1H-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_2H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_2H.1A01 AUG29_IKE_2H-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_2H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_2H.1B01 AUG29_IKE_2H-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_2H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_2H.2A01 AUG29_IKE_2H-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_2H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_2H.2B01 AUG29_IKE_2H-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_2H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_2H.3A01 AUG29_IKE_2H-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_2H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_2H.3B01 AUG29_IKE_2H-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_3H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_3H.1A01 AUG29_IKE_3H-1A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_3H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_3H.1B01 AUG29_IKE_3H-1B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_3H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_3H.2A01 AUG29_IKE_3H-2A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_3H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_3H.2B01 AUG29_IKE_3H-2B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_3H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_3H.3A01 AUG29_IKE_3H-3A01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG29_IKE_3H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry negative 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG29_IKE_3H.3B01 AUG29_IKE_3H-3B01.CDF Metabolite identification m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv

Assay  4

Assay file name: a_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
Technology: mass spectrometry
Platform: Liquid Chromatography MS - Positive (LC-MS (Positive))

Instrumentation

Sample Name Protocol REF Post Extraction Derivatization Extract Name Protocol REF Chromatography Instrument Column model Column type Labeled Extract Name Label Protocol REF Scan polarity Scan m/z range Instrument Ion source Mass analyzer MS Assay Name Raw Spectral Data File Protocol REF Normalization Name Derived Spectral Data File Protocol REF Data Transformation Name Metabolite Assignment File
AUG31.BLANK.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.BLANK.101 AUG31-BLANK-101.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.BLANK.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.BLANK.201 AUG31-BLANK-201.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.MATRIX.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.MATRIX.101 AUG31-MATRIX-101.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.MATRIX.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.MATRIX.201 AUG31-MATRIX-201.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.101 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.101 AUG31-QC-POS-101.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.201 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.201 AUG31-QC-POS-201.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.301 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.301 AUG31-QC-POS-301.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.401 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.401 AUG31-QC-POS-401.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.501 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.501 AUG31-QC-POS-501.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.601 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.601 AUG31-QC-POS-601.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.701 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.701 AUG31-QC-POS-701.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31.QC.POS.801 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31.QC.POS.801 AUG31-QC-POS-801.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_DMSO.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_DMSO.1A01 AUG31_DMSO-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_DMSO.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_DMSO.1B01 AUG31_DMSO-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_DMSO.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_DMSO.2A01 AUG31_DMSO-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_DMSO.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_DMSO.2B01 AUG31_DMSO-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_DMSO.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_DMSO.3A01 AUG31_DMSO-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_DMSO.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_DMSO.3B01 AUG31_DMSO-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_0H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_0H.1A01 AUG31_IKE_0H-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_0H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_0H.1B01 AUG31_IKE_0H-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_0H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_0H.2A01 AUG31_IKE_0H-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_0H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_0H.2B01 AUG31_IKE_0H-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_0H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_0H.3A01 AUG31_IKE_0H-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_0H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_0H.3B01 AUG31_IKE_0H-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_1H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_1H.1A01 AUG31_IKE_1H-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_1H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_1H.1B01 AUG31_IKE_1H-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_1H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_1H.2A01 AUG31_IKE_1H-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_1H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_1H.2B01 AUG31_IKE_1H-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_1H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_1H.3A01 AUG31_IKE_1H-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_1H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_1H.3B01 AUG31_IKE_1H-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_2H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_2H.1A01 AUG31_IKE_2H-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_2H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_2H.1B01 AUG31_IKE_2H-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_2H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_2H.2A01 AUG31_IKE_2H-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_2H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_2H.2B01 AUG31_IKE_2H-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_2H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_2H.3A01 AUG31_IKE_2H-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_2H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_2H.3B01 AUG31_IKE_2H-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_3H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_3H.1A01 AUG31_IKE_3H-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_3H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_3H.1B01 AUG31_IKE_3H-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_3H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_3H.2A01 AUG31_IKE_3H-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_3H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_3H.2B01 AUG31_IKE_3H-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_3H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_3H.3A01 AUG31_IKE_3H-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_3H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_3H.3B01 AUG31_IKE_3H-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_4H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_4H.1A01 AUG31_IKE_4H-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_4H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_4H.1B01 AUG31_IKE_4H-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_4H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_4H.2A01 AUG31_IKE_4H-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_4H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_4H.2B01 AUG31_IKE_4H-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_4H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_4H.3A01 AUG31_IKE_4H-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_4H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_4H.3B01 AUG31_IKE_4H-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_6H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_6H.1A01 AUG31_IKE_6H-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_6H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_6H.1B01 AUG31_IKE_6H-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_6H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_6H.2A01 AUG31_IKE_6H-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_6H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_6H.2B01 AUG31_IKE_6H-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_6H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_6H.3A01 AUG31_IKE_6H-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_6H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_6H.3B01 AUG31_IKE_6H-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_24H.1A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_24H.1A01 AUG31_IKE_24H-1A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_24H.1B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_24H.1B01 AUG31_IKE_24H-1B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_24H.2A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_24H.2A01 AUG31_IKE_24H-2A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_24H.2B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_24H.2B01 AUG31_IKE_24H-2B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_24H.3A01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_24H.3A01 AUG31_IKE_24H-3A01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
AUG31_IKE_24H.3B01 Extraction 1300 μl Methanol/MTBE/Water, 1.25/4.25/1 (v/v/v) none Chromatography Waters ACQUITY UPLC I-Class System ACQUITY UPLC HSS T3 (1.8 µm, 2.1 mm x 100 mm; Waters) reverse phase Mass spectrometry positive 50-1600 Waters SYNAPT G2 HDMS System MS: electrospray ionization quadrupole time-of-flight AUG31_IKE_24H.3B01 AUG31_IKE_24H-3B01.CDF Data transformation Metabolite identification m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
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File
audit
IKE-500NM-2B-POS01.CDF
IKE-500NM-1A01.CDF
AUG31_IKE_2H-1B01.CDF
AUG31_IKE_0H-1B01.CDF
AUG31_IKE_24H-2B01.CDF
DMSO-3A-POS01.CDF
IKE_BME-3B01.CDF
IKE_FER-3B-POS01.CDF
SEP27-18-QC-NEG-701.CDF
SEP29-18-MATRIX-POS-201.CDF
IKE-1UM-3A01.CDF
IKE-1UM-1A01.CDF
AUG29_IKE_1H-1B01.CDF
AUG29_IKE_1H-2B01.CDF
IKE_BME-2B01.CDF
IKE_BME-2B-POS01.CDF
SEP29-18-MATRIX-POS-1A01.CDF
SEP27-18-QC-POS-301.CDF
SEP27-18-QC-POS-201.CDF
AUG31_IKE_24H-2A01.CDF
IKE-500NM-3B01.CDF
AUG31_IKE_0H-3B01.CDF
AUG29_IKE_3H-1B01.CDF
AUG29_IKE_0H-3A01.CDF
IKE_DFO-3A01.CDF
i_Investigation.txt
IKE-500NM-1B01.CDF
IKE_FER-3B01.CDF
SEP27-18-QC-POS-701.CDF
IKE-1UM-2B-POS01.CDF
AUG31_DMSO-1A01.CDF
AUG29_IKE_6H-2B01.CDF
IKE_DFO-1A-POS01.CDF
IKE-1UM-2B01.CDF
SEP27-18-QC-POS-401.CDF
AUG31_IKE_1H-2B01.CDF
AUG29_IKE_0H-3B01.CDF
AUG31_IKE_4H-2B01.CDF
AUG29_IKE_0H-1A01.CDF
SEP29-18-BLANK-POS-201.CDF
SEP27-18-QC-POS-601.CDF
DMSO-3B-POS01.CDF
IKE-1UM-3A-POS01.CDF
IKE_FER-1A-POS01.CDF
AUG31_IKE_24H-1A01.CDF
AUG31_IKE_1H-1A01.CDF
IKE_FER-3A-POS01.CDF
AUG29_IKE_3H-2A01.CDF
AUG31_IKE_1H-3A01.CDF
IKE_DFO-2B-POS01.CDF
IKE_DFO-1B-POS01.CDF
AUG31_IKE_24H-3B01.CDF
IKE-1UM-2A01.CDF
IKE_DFO-1A01.CDF
AUG29-BLANK-201.CDF
AUG29_DMSO-3B01.CDF
AUG29-BLANK-101.CDF
IKE-500NM-3B-POS01.CDF
IKE_DFO-1B01.CDF
IKE-1UM-1B01.CDF
IKE-500NM-1B-POS01.CDF
IKE-1UM-3B01.CDF
SEP27-18-QC-NEG-601.CDF
IKE-500NM-2A01.CDF
IKE_DFO-3B01.CDF
IKE_BME-2A01.CDF
SEP27-18-QC-POS-501.CDF
SEP27-18-MATRIX-NEG-201.CDF
AUG29_IKE_1H-1A01.CDF
IKE_BME-3B-POS01.CDF
IKE_DFO-2A01.CDF
IKE_BME-1B-POS01.CDF
IKE_DFO-3A-POS01.CDF
SEP27-18-QC-NEG-201.CDF
IKE-500NM-2A-POS01.CDF
AUG31_IKE_1H-2A01.CDF
IKE_FER-3A01.CDF
IKE_BME-1A-POS01.CDF
AUG31_IKE_24H-1B01.CDF
AUG31_IKE_1H-3B01.CDF
IKE_BME-1B01.CDF
SEP27-18-QC-NEG-301.CDF
AUG31_IKE_2H-1A01.CDF
DMSO-2B01.CDF
IKE-1UM-1B-POS01.CDF
IKE-1UM-2A-POS01.CDF
AUG31_IKE_24H-3A01.CDF
IKE_DFO-3B-POS01.CDF
AUG29_IKE_1H-2A01.CDF
IKE-500NM-3A-POS01.CDF
AUG31_IKE_4H-2A01.CDF
AUG31_IKE_0H-2A01.CDF
AUG31_IKE_4H-3A01.CDF
DMSO-3B01.CDF
DMSO-2B-POS01.CDF
AUG31_IKE_0H-3A01.CDF
IKE_BME-1A01.CDF
IKE-500NM-3A01.CDF
AUG31-QC-POS-801.CDF
IKE_DFO-2B01.CDF
IKE_BME-2A-POS01.CDF
AUG31_IKE_4H-3B01.CDF
AUG29_IKE_6H-3A01.CDF
AUG31_IKE_1H-1B01.CDF
IKE_DFO-2A-POS01.CDF
DMSO-3A01.CDF
IKE-1UM-3B-POS01.CDF
SEP29-18-MATRIX-POS-101.CDF
AUG29_IKE_0H-2B01.CDF
IKE_FER-2B-POS01.CDF
AUG31_IKE_0H-2B01.CDF
IKE-500NM-2B01.CDF
SEP27-18-QC-NEG-101.CDF
IKE-1UM-1A-POS01.CDF
IKE-500NM-1A-POS01.CDF
SEP27-18-MATRIX-NEG-101.CDF
AUG29_IKE_1H-3A01.CDF
AUG29_DMSO-1A01.CDF
AUG29_DMSO-1B01.CDF
AUG29_DMSO-2A01.CDF
AUG29_DMSO-2B01.CDF
AUG29_DMSO-3A01.CDF
AUG29_IKE_0H-1B01.CDF
AUG29_IKE_0H-2A01.CDF
AUG29_IKE_1H-3B01.CDF
AUG29_IKE_24H-1A01.CDF
AUG29_IKE_24H-1B01.CDF
AUG29_IKE_24H-2A01.CDF
AUG29_IKE_24H-2B01.CDF
AUG29_IKE_24H-3A01.CDF
AUG29_IKE_24H-3B01.CDF
AUG29_IKE_2H-1A01.CDF
AUG29_IKE_2H-1B01.CDF
DMSO-1A01.CDF
AUG29_IKE_2H-2A01.CDF
AUG29_IKE_2H-2B01.CDF
AUG29_IKE_2H-3A01.CDF
AUG29_IKE_2H-3B01.CDF
AUG29_IKE_3H-1A01.CDF
AUG29_IKE_3H-2B01.CDF
AUG29_IKE_3H-3A01.CDF
AUG29_IKE_3H-3B01.CDF
AUG29_IKE_4H-1A01.CDF
AUG29_IKE_4H-1B01.CDF
AUG29_IKE_4H-2A01.CDF
AUG29_IKE_4H-2B01.CDF
AUG29_IKE_4H-3A01.CDF
AUG29_IKE_4H-3B01.CDF
AUG29_IKE_6H-1A01.CDF
AUG29_IKE_6H-1B01.CDF
AUG29_IKE_6H-2A01.CDF
AUG29_IKE_6H-3B01.CDF
AUG29-MATRIX-101.CDF
AUG29-MATRIX-1A01.CDF
AUG29-MATRIX-201.CDF
AUG29-QC-NEG-101.CDF
AUG29-QC-NEG-201.CDF
AUG29-QC-NEG-301.CDF
AUG29-QC-NEG-401.CDF
AUG29-QC-NEG-501.CDF
AUG29-QC-NEG-601.CDF
AUG29-QC-NEG-701.CDF
AUG29-QC-NEG-801.CDF
AUG31-BLANK-101.CDF
AUG31-BLANK-201.CDF
AUG31_DMSO-1B01.CDF
AUG31_DMSO-2A01.CDF
AUG31_DMSO-2B01.CDF
AUG31_DMSO-3A01.CDF
AUG31_DMSO-3B01.CDF
AUG31_IKE_0H-1A01.CDF
AUG31_IKE_2H-2A01.CDF
AUG31_IKE_2H-2B01.CDF
AUG31_IKE_2H-3A01.CDF
AUG31_IKE_2H-3B01.CDF
AUG31_IKE_3H-1A01.CDF
AUG31_IKE_3H-1B01.CDF
AUG31_IKE_3H-2A01.CDF
AUG31_IKE_3H-2B01.CDF
AUG31_IKE_3H-3A01.CDF
AUG31_IKE_3H-3B01.CDF
AUG31_IKE_4H-1A01.CDF
AUG31_IKE_4H-1B01.CDF
AUG31_IKE_6H-1A01.CDF
AUG31_IKE_6H-1B01.CDF
AUG31_IKE_6H-2A01.CDF
AUG31_IKE_6H-2B01.CDF
AUG31_IKE_6H-3A01.CDF
AUG31_IKE_6H-3B01.CDF
AUG31-MATRIX-101.CDF
AUG31-MATRIX-201.CDF
AUG31-QC-POS-101.CDF
AUG31-QC-POS-201.CDF
AUG31-QC-POS-301.CDF
AUG31-QC-POS-401.CDF
AUG31-QC-POS-501.CDF
AUG31-QC-POS-601.CDF
AUG31-QC-POS-701.CDF
DMSO-1A-POS01.CDF
DMSO-1B01.CDF
DMSO-1B-POS01.CDF
DMSO-2A01.CDF
DMSO-2A-POS01.CDF
IKE_BME-3A01.CDF
IKE_BME-3A-POS01.CDF
IKE_FER-1A01.CDF
IKE_FER-1B01.CDF
IKE_FER-1B-POS01.CDF
IKE_FER-2A01.CDF
IKE_FER-2A-POS01.CDF
IKE_FER-2B01.CDF
SEP27-18-QC-NEG-401.CDF
SEP27-18-QC-NEG-501.CDF
SEP27-18-QC-POS-101.CDF
metexplore_mapping.json
IKE-Ferroptosis-pos-neg_archive.zip
IKE-Ferroptosis-pos-neg_archive
a_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
a_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
a_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
a_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry.txt
s_IKE-Ferroptosis.txt
m_cell_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
m_cell_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
m_xeno_neg_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv
m_xeno_pos_ike-ferroptosis_metabolite_profiling_mass_spectrometry_v2_maf.tsv

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Validations marked with (*) have been allowed by the MetaboLights Curators.
Click here for the detailed description of Validations.
Condition Status Description Requirement Group Message
PASSES Study Title MANDATORY STUDY OK
PASSES Study Description MANDATORY STUDY OK
PASSES Study text successfully parsed OPTIONAL STUDY OK
PASSES Study Contact(s) have listed email MANDATORY CONTACT OK
PASSES Sample(s) MANDATORY SAMPLES OK
PASSES Sample Name consistency check MANDATORY ASSAYS OK
PASSES Publication(s) associated with this Study MANDATORY PUBLICATION OK
PASSES Minimal Experimental protocol MANDATORY PROTOCOLS OK
PASSES Comprehensive Experimental protocol OPTIONAL PROTOCOLS OK
PASSES Extraction protocol description MANDATORY PROTOCOLS OK
PASSES Data transformation protocol description MANDATORY PROTOCOLS OK
PASSES Metabolite Identification protocol description MANDATORY PROTOCOLS OK
PASSES Mass spectrometry protocol description MANDATORY PROTOCOLS OK
PASSES Chromatography protocol description MANDATORY PROTOCOLS OK
PASSES Sample Collection protocol description MANDATORY PROTOCOLS OK
PASSES Protocols text successfully parsed OPTIONAL PROTOCOLS OK
PASSES Organism name MANDATORY ORGANISM OK
PASSES Organism part MANDATORY ORGANISM OK
PASSES Study Factors MANDATORY FACTORS OK
PASSES Assay platform information OPTIONAL ASSAYS OK
PASSES Assay has raw files referenced MANDATORY FILES OK
PASSES Assay referenced raw files detection in filesystem MANDATORY FILES OK
PASSES Raw files in the Assay(s) have the correct format MANDATORY FILES OK
PASSES Assay(s) MANDATORY ASSAYS OK
PASSES All Assays have Metabolite Assignment File (MAF) referenced OPTIONAL FILES OK
PASSES Metabolite Assignment File (MAF) is present in Study folder MANDATORY FILES OK
PASSES Metabolite Assignment File (MAF) has correct format MANDATORY FILES OK
PASSES Metabolite Identification File (MAF) content MANDATORY FILES OK
PASSES ISA-Tab investigation file check MANDATORY ISATAB OK

Pathways - Assay  1



MetExplore Pathways Mapping

Name DB Identifier Mapped Metabolite(s)

Pathways - Assay  2



MetExplore Pathways Mapping

Name DB Identifier Mapped Metabolite(s)

Pathways - Assay  3



MetExplore Pathways Mapping

Name DB Identifier Mapped Metabolite(s)

Pathways - Assay  4



MetExplore Pathways Mapping

Name