$VAR1 = undef;

Summary for peptidase C01.001: papain

Names
MEROPS Name	papain
Other names	papaya peptidase I

Domain architecture

MEROPS Classification
Classification	Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.001
Holotype	papain (Carica papaya), Uniprot accession P00784 (peptidase unit: 134-345), MERNUM MER0000647
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Cysteine
Peplist	Included in the Peplist with identifier PL00046
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.2
Enzymology	BRENDA database
Proteolytic events	CutDB database (3 cleavages)
Biotechnology	Used in food processing.
Physiology	Believed to confer resistance to pests on the source plants.
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/Papain
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/-/l/rQ/Q/-/D (based on 89 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	3	3	1	6	4	4	4	3
Pro	2	9	2	1	0	4	3	4
Ala	3	3	3	4	7	1	2	1
Val	1	2	6	2	3	1	1	3
Leu	4	7	36	6	4	3	4	2
Ile	1	1	1	1	1	2	0	1
Met	0	1	3	4	1	0	0	0
Phe	1	2	10	10	2	2	2	1
Tyr	1	1	4	3	2	2	2	2
Trp	0	0	1	1	0	0	0	0
Ser	1	2	1	2	3	2	1	1
Thr	1	0	5	4	2	2	0	1
Cys	1	0	1	2	0	1	1	0
Asn	1	0	1	3	1	1	0	1
Gln	0	1	1	2	46	42	0	0
Asp	1	1	1	2	1	0	0	46
Glu	10	2	3	2	2	10	4	3
Lys	0	1	2	1	1	0	3	9
Arg	2	1	2	28	0	3	9	1
His	2	1	2	3	1	2	1	1

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Carica papaya	wild type	P	P	V	R/Q/A	A/S/T	S/R	G/K/P/R	P/Q/N	PPVRASGP	Abz-Tyr(NO2)	St Hilaire et al., 1999
Carica papaya	wild type	P/G/H/broad	P/R	V	R/K	-	-	-	-	PPVR	ACC	Choe et al., 2006
Carica papaya	wild type	-	-	-	K	-	-	-	-	xxxKxxxx	ACC-Dabcyl	Sun et al., 2007
Carica papaya	wild type	-	-	-	Iaf/R	-	-	-	-	xxxIafxxxx	Abz-EDDnp	Alves et al., 2001
Carica papaya	wild type	-	-	-	-	L/A/S/F	-	-	-	xxxxLxxx	Dansyl-Trp	M�nard et al., 1993
Carica papaya	wild type	-	-	-	-	L	-	-	-	xxxxLxxx	Abz-EDDnp	Melo et al., 2001
Carica papaya	wild type	-	-	-	G	-	-	-	-	xxxGxxxx	Abz-EDDnp	Del Nery et al., 2000