| Family type peptidase | S10.001 - carboxypeptidase Y (Saccharomyces cerevisiae), MEROPS Accession MER0002010 (peptidase unit: 112-532) |
| Content of family | Peptidase family S10 contains only carboxypeptidases. |
| History |
Identifier created: Biochem.J. 290:205-218 (1993)
|
| Catalytic type | Serine |
| Active site residues | S257 D449 H508 |
| Active site | The residues of the catalytic triad in family S10 occur in the order Ser, Asp and His (Jung et al., 1998). There is a conserved Glu preceding the catalytic serine that is thought to be responsible for the acidic pH optimum of the carboxypeptidases. |
| Activities and specificities | The peptidase family differs from most other serine peptidase families (S53 being the exception) in that its peptidases are only active at acidic pH. The carboxypeptidases in family S10 show two main types of specificity. Some (e.g. carboxypeptidase C) show a preference for hydrophobic residues in positions P1 and P1". Carboxypeptidases of the second set (e.g. carboxypeptidase D) display a preference for the basic amino acids either side of the scissile bond, but are also able to cleave peptides with hydrophobic residues in these positions. It is notable that carboxypeptidases specific for hydrophobic or basic residues are also found among the metallocarboxypeptidases in family M14. |
| Inhibitors | Natural inhibitors include a high affinity inhibitor of carboxypeptidase Y encoded by the TFSI gene of Saccharomyces cerevisiae . Antipain and chymostatin inhibit carboxypeptidase D (Bullock et al., 1996). The serine carboxypeptidases are also inhibited by DFP and PMSF. |
| Molecular structure | The peptidases of family S10 have what has been termed the alpha/beta hydrolase fold (Ollis et al., 1992). Family S10 peptidases contain 14 alpha helices and 11 mixed beta sheets; the six central beta strands are surrounded on either side by the 14 alpha helices. The catalytic triads of carboxypeptidase C, chymotrypsin and subtilisin can be superimposed, even though the folds are unrelated. |
| Clan | SC |
| Basis of clan assignment | Type family of clan SC. |
| Peptidases and Homologues |
MEROPS ID |
Structure |
| carboxypeptidase Y | S10.001 | Yes |
| serine carboxypeptidase A | S10.002 | Yes |
| vitellogenic carboxypeptidase-like protein | S10.003 | - |
| serine carboxypeptidase C | S10.004 | - |
| serine carboxypeptidase D | S10.005 | Yes |
| Mername-AA083 peptidase | S10.006 | - |
| kex carboxypeptidase | S10.007 | Yes |
| carboxypeptidase OcpA | S10.008 | - |
| serine carboxypeptidase III (plant) | S10.009 | - |
| serine carboxypeptidase Z (Absidia zachae) and similar | S10.010 | - |
| serine carboxypeptidase P | S10.011 | - |
| Sxa2 carboxypeptidase | S10.012 | - |
| SCPEP1 peptidase | S10.013 | - |
| carboxypeptidase O | S10.014 | - |
| BRS1 serine carboxypeptidase | S10.015 | - |
| carboxypeptidase OcpB | S10.016 | - |
| OsBISCPL1-type putative carboxypeptidase | S10.017 | - |
| serine carboxypeptidase 46 (Oryza sativa) | S10.018 | - |
| At1g33540 (Arabidopsis thaliana) | S10.A01 | - |
| At3g12230 (Arabidopsis thaliana) | S10.A02 | - |
| At1g73290 (Arabidopsis thaliana) | S10.A03 | - |
| At1g73270 (Arabidopsis thaliana) | S10.A04 | - |
| At1g73280 (Arabidopsis thaliana) | S10.A05 | - |
| At1g73310 (Arabidopsis thaliana) | S10.A06 | - |
| At5g36180 (Arabidopsis thaliana) | S10.A07 | - |
| At2g22970 (Arabidopsis thaliana) | S10.A08 | - |
| At2g22920 (Arabidopsis thaliana) | S10.A09 | - |
| At3g25420 (Arabidopsis thaliana) | S10.A10 | - |
| At4g12910 (Arabidopsis thaliana) | S10.A11 | - |
| At2g23010 (Arabidopsis thaliana) | S10.A12 | - |
| At2g23000 (Arabidopsis thaliana) | S10.A13 | - |
| At2g22990 (Arabidopsis thaliana) | S10.A14 | Yes |
| At3g10450 (Arabidopsis thaliana) | S10.A15 | - |
| At1g73300 (Arabidopsis thaliana) | S10.A16 | - |
| At3g12220 (Arabidopsis thaliana) | S10.A17 | - |
| At5g09640 (Arabidopsis thaliana) | S10.A18 | Yes |
| At3g12203 (Arabidopsis thaliana) | S10.A19 | - |
| At3g17180 (Arabidopsis thaliana) | S10.A20 | - |
| At5g42240 (Arabidopsis thaliana)-type peptidase | S10.A21 | - |
| At5g42230 (Arabidopsis thaliana) | S10.A22 | - |
| At3g07990 (Arabidopsis thaliana)-type peptidase | S10.A23 | - |
| At1g28110 (Arabidopsis thaliana)-type peptidase | S10.A24 | - |
| At1g43780 (Arabidopsis thaliana) | S10.A25 | - |
| At1g61130 (Arabidopsis thaliana) | S10.A26 | - |
| At4g15100 (Arabidopsis thaliana) | S10.A27 | - |
| At2g35770 (Arabidopsis thaliana) | S10.A28 | - |
| At2g05850 (Arabidopsis thaliana) | S10.A29 | - |
| At2g24010 (Arabidopsis thaliana) | S10.A30 | - |
| At3g52010 (Arabidopsis thaliana) | S10.A31 | - |
| At4g30810 (Arabidopsis thaliana) | S10.A32 | - |
| At2g12480 (Arabidopsis thaliana) | S10.A33 | - |
| At5g08260 (Arabidopsis thaliana) | S10.A34 | - |
| At2g24000 (Arabidopsis thaliana) | S10.A35 | - |
| At1g11080 (Arabidopsis thaliana) | S10.A36 | - |
| At3g52000 (Arabidopsis thaliana) | S10.A37 | - |
| At3g52020 (Arabidopsis thaliana) | S10.A38 | - |
| At5g23210 (Arabidopsis thaliana) | S10.A39 | - |
| At3g02110 (Arabidopsis thaliana) | S10.A40 | - |
| At3g63470 (Arabidopsis thaliana) | S10.A41 | - |
| At2g33530 (Arabidopsis thaliana) | S10.A42 | - |
| At2g35780 (Arabidopsis thaliana) | S10.A43 | Yes |
| At5g22980 (Arabidopsis thaliana) | S10.A44 | - |
| At3g10410 (Arabidopsis thaliana) | S10.A45 | - |
| At3g45010 (Arabidopsis thaliana) | S10.A46 | - |
| At2g22960 (Arabidopsis thaliana) | S10.A47 | - |
| At3g56540 (Arabidopsis thaliana) | S10.A48 | - |
| putative serine carboxypeptidase (Saccharomyces cerevisiae) | S10.A49 | - |
| CG31821 (Drosophila melanogaster) | S10.A50 | - |
| CG31823 protein (Drosophila melanogaster) | S10.A51 | - |
| CG32483 protein (Drosophila melanogaster) | S10.A52 | - |
| CG3344 protein (Drosophila melanogaster) | S10.A53 | - |
| Y40D12A.2 g.p. (Caenorhabditis elegans) | S10.A54 | - |
| F13D12.6 g.p. (Caenorhabditis elegans) | S10.A55 | - |
| C08H9.1 g.p. (Caenorhabditis elegans) | S10.A56 | - |
| Y16B4A.2 g.p. unit 3 (Caenorhabditis elegans) | S10.A57 | - |
| K10B2.2 g.p. (Caenorhabditis elegans) | S10.A58 | - |
| F41C3.5 g.p. (Caenorhabditis elegans) | S10.A59 | - |
| K10C2.1 g.p. (Caenorhabditis elegans) | S10.A60 | - |
| Y32F6A.5 g.p. (Caenorhabditis elegans) | S10.A61 | - |
| Y16B4A.2 g.p. (Caenorhabditis elegans) | S10.A62 | - |
| F22E12.1 g.p. (Caenorhabditis elegans) | S10.A63 | - |
| K10C2.1 g.p. (Caenorhabditis elegans) | S10.A64 | - |
| F32A5.3 (Caenorhabditis elegans) | S10.A65 | - |
| cpy1 g.p. (Schizosaccharomyces pombe) | S10.A66 | - |
| kex1 g.p. (Schizosaccharomyces pombe) | S10.A67 | - |
| DDB_G0280105 g.p. (Dictyostelium discoideum) | S10.A68 | - |
| SCPL54 g.p. (Arabidopsis thaliana) | S10.A69 | - |
| Family S10 non-peptidase homologues | non-peptidase homologue | - |
| Family S10 unassigned peptidases | unassigned | - |
