|Family type peptidase||C76.001 - viral tegument protein deubiquitinylating peptidase (human herpesvirus 1), MEROPS Accession MER0045029 (peptidase unit: 49-221)|
|Content of family||Peptidase family C76 contains ubiquitinyl hydrolases.|
Identifier created: Merops 7.2 (14 October 2005)|
Ubiquitinyl hydrolase activity was discovered within an N-terminal fragment of the UL36 protein from herpes simpex virus (C76.001; Kattenhorn et al., 2005).
|Active site residues||C65 H199 |
|Active site||The catalytic Cys65 has been identified by site-directed mutagenesis (Kattenhorn et al., 2005). The second member of a catalytic dyad may be the completely conserved His199.|
|Activities and specificities||The enzyme from herpes simplex virus is specific for release of ubiquitin from proteins ubiquitinylated via Lys48. Activity is only detectable following cleavage of the UL36 protein, which occurs late during viral replication. Activity has been shown towards ubiquitin-aminomethylcoumarin, ubiquitin-branched peptides and poly-ubiquitin (Kattenhorn et al., 2005).|
|Molecular structure||The tertiary structure of UL36 from murine herpesvirus 1 has been solved (Schlieker et al., 2007) and show a fold similar to that of papain (C01.001). Consequently, this family is included in clan CA.|