Family C59
Summary for family C59
| Family type peptidase | C59.001 - penicillin V acylase precursor (Lysinibacillus sphaericus), MEROPS Accession MER0020029 (peptidase unit: 4-338) |
| Content of family | Peptidase family C59 contains Cys-dependent Ntn-hydrolases. |
| History |
Identifier created: MEROPS 6.4 (24 September 2003)
Cys-dependent Ntn-hydrolases are only classified as peptidases by virtue of their self-processing activity. The autolysis step produces a mature amidase with Cys1 as the nucleophile. All peptidase activity is lost on conversion to the mature peptidase. |
| Catalytic type | Cysteine |
| Active site residues | C4 |
| Active site | The catalytic Cys becomes the new N-terminus following autolysis (see the Alignment). |
| Activities and specificities | The only known peptidase activity is the autolytic cleavage of the penicillin V acylase precursor protein (C59.001). |
| Molecular structure | The mature peptidase is a homotetramer and has a fold that is similar to that of the proteasome (##XT01.001##). There are two anti-parallel beta sheets sandwiched between two pairs of anti-parallel alpha helices. There are extensions from one of the pairs of alpha helices and also at the C-terminus, which interact with other monomers in the tetramer and provide stability (Suresh et al., 1999). |
| Clan | PB |
| Subclan | PB(C) |
