{"metadata":{"accession":"Q67W82","id":"4CL4_ORYSJ","source_organism":{"taxId":"39947","scientificName":"Oryza sativa subsp. japonica","fullName":"Oryza sativa subsp. japonica (Rice)"},"name":"4-coumarate--CoA ligase 4","description":["Involved in the phenylpropanoid metabolism by mediating the activation of a number of hydroxycinnamates for the biosynthesis of monolignols and other phenolic secondary metabolites (PubMed:21807887, PubMed:23246835). Catalyzes the formation of CoA esters of cinnamate, 4-coumarate, caffeate and ferulate (PubMed:21807887, PubMed:23246835). Is more efficient with substrates in the following order: 4-coumarate > ferulate > caffeate > cinnamate (PubMed:21807887). Cannot convert sinapate to its corresponding CoA ester (PubMed:21807887, PubMed:23246835). Required for the biosynthesis of lignin in roots and shoots (PubMed:32989851). Follows a two-step reaction mechanism, wherein the carboxylate substrate first undergoes adenylation by ATP, followed by a thioesterification in the presence of CoA to yield the final CoA thioesters (By similarity)"],"length":559,"sequence":"MGSMAAAAEAAQEEETVVFRSKLPDIEIPSHLTLQAYCFEKLPEVAARPCLIDGQTGAVYSYGEVEELSRRAAAGLRRLGVGKGDVVMSLLRNCPEFAFTFLGAARLGAATTTANPFYTPHEIHRQASAAGARVIVTEACAVEKVRGFAADRGIPVVAVDGDFDGCVGFGEAMLDASIEPLDADEEVHPDDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGENPNLYFRREDVVLCLLPLFHIYSLNSVLLAGLRAGSAIVIMRKFDLGALVDLTRRHGVTVAPFVPPIVVEIAKSPRVTADDLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFAKEPFEVKSGSCGTVVRNAELKIVDPDTGATLGRNQSGEICIRGEQIMKGYLNDPESTKNTIDKGGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPDIKDAAVVPMIDEIAGEVPVAFIVRIEGSAISENEIKQFVAKEVVFYKRLNKVFFADSIPKSPSGKILRKDLRAKLAAGIPTNDNTQLKS","proteome":"UP000059680","gene":"4CL4","go_terms":null,"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"ba6ba0b0e99fc7c9ffcc323888fecbac8c6c4763","counters":{"domain_architectures":241937,"entries":13,"isoforms":2,"proteomes":1,"sets":3,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":2,"cdd":1,"ssf":1,"pfam":2,"panther":1,"prosite":1,"interpro":5},"proteome":1,"taxonomy":1,"similar_proteins":241937}}}