{"metadata":{"accession":"Q4WAY4","id":"FMAC_ASPFU","source_organism":{"taxId":"330879","scientificName":"Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)","fullName":"Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)"},"name":"Polyketide transferase af380","description":["Polyketide transferase; part of the gene cluster that mediates the biosynthesis of fumagillin, a meroterpenoid that has numerous biological activities including irreversible inhibition of human type 2 methionine aminopeptidase (METAP2) (PubMed:23488861, PubMed:24568283). Within the pathway, the polyketide transferase af380 catalyzes the transfer of a dodecapentaenoyl group synthesized by the polyketide synthase af370 onto 5R-hydroxy-seco-sesquiterpene to produce prefumagillin (PubMed:23488861). The pathway begins with the conversion of farnesyl pyrophosphate (FPP) to beta-trans-bergamotene by the membrane-bound beta-trans-bergamotene synthase af520. The multifunctional cytochrome P450 monooxygenase af510 then converts beta-trans-bergamotene into 5-keto-demethoxyfumagillol via several oxydation steps. 5-keto-demethoxyfumagillol is then subjected to successive C-6 hydroxylation and O-methylation by the dioxygenase af480 and O-methyltransferase af390-400, respectively, to yield 5-keto-fumagillol, which is then stereoselectively reduced by the keto-reductase af490 to 5R-hydroxy-seco-sesquiterpene. The next step is the polyketide transferase af380-catalyzed transfer of a dodecapentaenoyl group synthesized by the polyketide synthase af370 onto 5R-hydroxy-seco-sesquiterpene which leads to the production of prefumagillin. Finally, oxidative cleavage by the monooxygenase af470 converts prefumagillin to fumagillin (Probable) (PubMed:24568283)"],"length":292,"sequence":"MNREDVEFPTCDGLILRGWLYPGTIRGPAIVMNQGFNTPKEILLPDVAVWFQQQGVTVLLYDNRCIGASDGEPRNDVKPAKLVEDFHDALTFMARHPMVDEDKIILYGYSFSAMTALVAAGLDHRVGAAISVTPIANYDFREKEKNNVMALAMQDRVSTLAGNDPVYIPFVGDDGYNPAGWGNQYNMEQFRAFLGTSFFTNRTTVQSYYHVLAWQPYGAMRLIKGTPVMMVTPAEDTISSPADQRAVFDMIPEPNKEFDLVAGRGHMDVINGEGAEEVLQRQLAFMRKHLDF","proteome":"UP000002530","gene":"af380","go_terms":null,"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"fafa3009ac3227235dfd20e7216f45a8ae4f27e5","counters":{"domain_architectures":104078,"entries":8,"isoforms":0,"proteomes":1,"sets":1,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cathgene3d":2,"pfam":1,"panther":1,"interpro":3},"proteome":1,"taxonomy":1,"similar_proteins":104078}}}