{"metadata":{"accession":"P70687","id":"CP17A_MESAU","source_organism":{"taxId":"10036","scientificName":"Mesocricetus auratus","fullName":"Mesocricetus auratus (Golden hamster)"},"name":"Steroid 17-alpha-hydroxylase/17,20 lyase","description":["A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase)"],"length":511,"sequence":"MWELVALLLLTLAYFFWSKSKTCGAKSPKSLPFLPLVGSLPFIPRHGHPHVNFFKLQEKYGPIYSLRLGSTTTVIIGQYQLAKEVLVKKGKEFSGRPHMVTLGLLSDQGKGIAFADSGGSWQLHRKLALSSFALFRDGNQKLEKIICQKASSLCDFLLTHNEESIDLSEPIFNSITNIICIICFGISYENRDPILATIKSFTEGILNSLGNDHLVDIFPWLTIFPNKTVDMIKKNVKIRDEVLSGILEKCKEKFNSDSISSLMDLLIQAKTNADNNNTSEGQGSNAFSDMHILATIADIFGAGIETTASVLSWIIAFLLHNPEVKKKIQKEIDQNIGFSRTPTFNDRNHLLMLEATIREVLRIRPVAPMLIPHRANSDMSIGEFSIPKFTPVIINLWALHHSEKEWDQPDRFMPERFLDPTGSHLITPSLSYLPFGAGARSCIGEVLARQELFLFMAHLLQRFDLDVPDDEQPPCLKGNANVVFLIDPFKVKITVRQAWKDAQAEVNTWRP","proteome":"UP000886700","gene":"CYP17A1","go_terms":[{"identifier":"GO:0004497","name":"monooxygenase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005506","name":"iron ion binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016705","name":"oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0020037","name":"heme binding","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":2,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"a02e61ba6c64bb9a9c779746e03a6c43264a16fb","counters":{"domain_architectures":520850,"entries":12,"isoforms":0,"proteomes":1,"sets":1,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"pfam":1,"ssf":1,"cdd":1,"panther":1,"prints":2,"prosite":1,"interpro":4},"proteome":1,"taxonomy":1,"similar_proteins":520850}}}