{"metadata":{"accession":"P49996","id":"DNAA_VIBHA","source_organism":{"taxId":"669","scientificName":"Vibrio harveyi","fullName":"Vibrio harveyi"},"name":"Chromosomal replication initiator protein DnaA","description":["Plays an essential role in the initiation and regulation of chromosomal replication. ATP-DnaA binds to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex once per cell cycle. Binds the DnaA box (a 9 base pair repeat at the origin) and separates the double-stranded (ds)DNA. Forms a right-handed helical filament on oriC DNA; dsDNA binds to the exterior of the filament while single-stranded (ss)DNA is stabiized in the filament's interior. The ATP-DnaA-oriC complex binds and stabilizes one strand of the AT-rich DNA unwinding element (DUE), permitting loading of DNA polymerase. After initiation quickly degrades to an ADP-DnaA complex that is not apt for DNA replication. Binds acidic phospholipids","Complements a temperature-sensitive E.coli mutant, the DnaA consensus is 5'-TT(A/T)TNCACA-3' (PubMed:11948168)"],"length":468,"sequence":"MSSSLWLQCLQQLQEELPATEFSMWVRPLQAELNDNTLTLFAPNRFVLDWVRDKYLNSINRLLQEYCGNDVPSLRFEVGSRRVAAPKPAPTRTPADVAAESSAPAQLQARKPVHKTWDDDAQVIADINHRSNVNPKHKFNNFVEGKSNQLGLAAARQVSDNPGAAYNPLFLYGGTGLGKTHLLHAVGNAIVDNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEISGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKAEDHQIHLADEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQLAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDYSNLIRTLSS","proteome":null,"gene":"dnaA","go_terms":[{"identifier":"GO:0043565","name":"sequence-specific DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005524","name":"ATP binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006270","name":"DNA replication initiation","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0006275","name":"regulation of DNA replication","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0003677","name":"DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003688","name":"DNA replication origin binding","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":2,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"3682acfbe4eaffe58dc72fb75756cc1201c63ea4","counters":{"domain_architectures":964,"entries":30,"isoforms":0,"proteomes":0,"sets":6,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":4,"pfam":4,"ssf":2,"cdd":2,"smart":2,"panther":1,"hamap":1,"ncbifam":1,"prints":1,"prosite":1,"interpro":11},"proteome":0,"taxonomy":1,"similar_proteins":964}}}