{"metadata":{"accession":"P0AEJ4","id":"ENVZ_ECOLI","source_organism":{"taxId":"83333","scientificName":"Escherichia coli (strain K12)","fullName":"Escherichia coli (strain K12)"},"name":"Sensor histidine kinase EnvZ","description":["Member of the two-component regulatory system EnvZ/OmpR involved in osmoregulation (particularly of genes ompF and ompC) as well as other genes (PubMed:2997120, PubMed:3536870). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals; at low osmolarity OmpR activates ompF transcription, while at high osmolarity it represses ompF and activates ompC transcription (PubMed:1323560, PubMed:2277041, PubMed:2558046, PubMed:2656684, PubMed:2668281, PubMed:2668953, PubMed:2674113). Also dephosphorylates OmpR in the presence of ATP (PubMed:1323560, PubMed:2277041, PubMed:2558046, PubMed:2668281). The cytoplasmic dimerization domain (CDD) forms an osmosensitive core; increasing osmolarity stabilizes this segment (possibly by its contraction), enhancing the autophosphorylation rate and consequently, downstream phosphotransfer to OmpR and signaling (PubMed:22543870, PubMed:28256224). Autophosphorylation is greater when full-length EnvZ is reconstituted in a lipid environment, lipid-mediated allostery impacts the kinase function of EnvZ (PubMed:28256224). Involved in acid stress response; this requires EnvZ but not OmpR phosphorylation, and suggests that EnvZ senses cytoplasmic acidic pH (PubMed:29138484)"],"length":450,"sequence":"MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG","proteome":"UP000000625","gene":"envZ","go_terms":[{"identifier":"GO:0007165","name":"signal transduction","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0016020","name":"membrane","category":{"code":"C","name":"cellular_component"}},{"identifier":"GO:0000155","name":"phosphorelay sensor kinase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016772","name":"transferase activity, transferring phosphorus-containing groups","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016310","name":"phosphorylation","category":{"code":"P","name":"biological_process"}}],"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"ed5ddfc4745162f183328622157829c334589c3b","counters":{"domain_architectures":119765,"entries":26,"isoforms":0,"proteomes":1,"sets":5,"structures":11,"taxa":1,"dbEntries":{"pfam":3,"cathgene3d":2,"profile":2,"smart":3,"cdd":3,"ssf":2,"ncbifam":1,"panther":1,"prints":1,"interpro":8},"proteome":1,"taxonomy":1,"similar_proteins":119765}}}