{"metadata":{"accession":"P07012","id":"RF2_ECOLI","source_organism":{"taxId":"83333","scientificName":"Escherichia coli (strain K12)","fullName":"Escherichia coli (strain K12)"},"name":"Peptide chain release factor RF2","description":["Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (PubMed:11118225, PubMed:17932046). Acts as a peptidyl-tRNA hydrolase (PubMed:22857598, PubMed:27934701). In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center (PTC) and releases the ribosome (PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Recruited by ArfA to rescue stalled ribosomes in the absence of a normal stop codon (PubMed:22857598, PubMed:22922063, PubMed:25355516). A TnaC-stalled ribosome binds RF2, but the active GGQ motif is prevented from engaging with the PTC by TnaC (PubMed:34504068)"],"length":365,"sequence":"MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRTSGAGGQHVNRTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEASLKAGL","proteome":"UP000000625","gene":"prfB","go_terms":[{"identifier":"GO:0006415","name":"translational termination","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0003747","name":"translation release factor activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016149","name":"translation release factor activity, codon specific","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005737","name":"cytoplasm","category":{"code":"C","name":"cellular_component"}}],"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"097b3b736c3d8a49ca7d8f8a450b23ec77e9df60","counters":{"domain_architectures":58959,"entries":15,"isoforms":0,"proteomes":1,"sets":1,"structures":28,"taxa":1,"dbEntries":{"cathgene3d":3,"ssf":1,"pfam":2,"smart":1,"hamap":1,"panther":1,"ncbifam":1,"prosite":1,"interpro":4},"proteome":1,"taxonomy":1,"similar_proteins":58959}}}