{"metadata":{"accession":"P00959","id":"SYM_ECOLI","source_organism":{"taxId":"83333","scientificName":"Escherichia coli (strain K12)","fullName":"Escherichia coli (strain K12)"},"name":"Methionine--tRNA ligase","description":["Catalyzes the attachment of L-methionine to tRNA(Met) (PubMed:216545, PubMed:367445, PubMed:4297674, PubMed:4581817, PubMed:7932711). Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (PubMed:216545, PubMed:4581817). MetRS can indeed attach methionine to tRNA(mMet), which supplies methionine for elongation of nascent peptides, and to tRNA(fMet), which supplies formylmethionine for initiation of peptide synthesis (PubMed:216545, PubMed:4581817). However, MetRS charges tRNA(fMet) with a greater efficiency than tRNA(mMet) in several strains of E.coli, suggesting a preference for initiation of new peptides (PubMed:216545). Cannot use D-methionine (PubMed:4297674)","In addition, MetRS can reject misactivated homocysteine by a tRNA-independent hydrolysis of the homocysteinyl-AMP intermediate, preventing incorporation of homocysteine into tRNA and proteins (PubMed:2191291, PubMed:7024910). The homocysteinyl-AMP intermediate undergoes an intramolecular cyclization reaction in which the thiolate side chain of homocysteine displaces the AMP group, forming homocysteine-thiolactone and AMP (PubMed:2191291, PubMed:7024910)"],"length":677,"sequence":"MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGHEVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEENRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCEVCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSVSFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKMSKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLNTELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDPIQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK","proteome":"UP000000625","gene":"metG","go_terms":[{"identifier":"GO:0004825","name":"methionine-tRNA ligase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006431","name":"methionyl-tRNA aminoacylation","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0000166","name":"nucleotide binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0004812","name":"aminoacyl-tRNA ligase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005524","name":"ATP binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006418","name":"tRNA aminoacylation for protein translation","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0000049","name":"tRNA binding","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"605e59fa9284a45ce9616dfe8a2213f70331198f","counters":{"domain_architectures":13265,"entries":34,"isoforms":0,"proteomes":1,"sets":6,"structures":24,"taxa":1,"dbEntries":{"ssf":4,"cdd":3,"cathgene3d":4,"pfam":3,"profile":1,"panther":1,"ncbifam":3,"hamap":1,"prosite":1,"prints":1,"interpro":12},"proteome":1,"taxonomy":1,"similar_proteins":13265}}}