{"metadata":{"accession":"O27661","id":"TOP1_METTH","source_organism":{"taxId":"187420","scientificName":"Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)","fullName":"Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)"},"name":"DNA topoisomerase 1","description":["Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone"],"length":718,"sequence":"MRSLSGKMHEVIICEKPKSSEKIAGALFPDAMKKKHGKVSYWEHVEGDKRVTIVSAVGHLYSLRPRQSNEEHFFDLEWAPIHEIDKKKGYVKDYLNVIRKFAAGADRYIHACDYDIEGTLIGFNALKYGCGEEALRKTSRMKFSTLTREEIQRAYKNPIEVDYGQVDSGAARHILDFIFGVNISRSLMKSVKAATNRFIKLSAGRVQTPTLAILVEREKEIRDFKPVPYWIIRAELGEGIIAESKRGKIFKRELVDSILEKCQGSDAEVKDVRVRDTIRKPPVPFDLGTLQSEAYRVFGFSPKKTQTIAQNLYTEGYTSYPRTSSQKLPESIGYEKILKNLAKNPRFGVHIERLRGPLKPHEGKKEDDAHPAIHPTGLLPSELSKDEKKVYDLIVHRFISVFGEDAILQTMKVELEIGEEEFSFSRKRVSKAGWMESYPYTKMEDEEFPEISGGDSLAVRSVSADERETKPPARYNEASLIRELERRGLGTKSTRADIIAKLYDRKYIEGKKIRVSPLGENIIDTLTRYCEKIISEELTRQFERELEDIMRGKISKDRVIDEAITEVRSILSDIEENLRDIGKELYRAYQDSRVVGECPACGGKLVIKYSPRNRSTFVGCSSYPDCRTVYSLPRGASVLKSLCEKCGLPMISYGRPRQRACLDPKCGKKKSEVEEVVGKCPECGSDLIKRSGRYGEFVGCKGFPKCRFTCSVDEVPEG","proteome":"UP000005223","gene":"topA","go_terms":[{"identifier":"GO:0003677","name":"DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003916","name":"DNA topoisomerase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006265","name":"DNA topological change","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0005694","name":"chromosome","category":{"code":"C","name":"cellular_component"}},{"identifier":"GO:0003917","name":"DNA topoisomerase type I (single strand cut, ATP-independent) activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"11c2314b289f96f2d423a6c34998a980f58fa56e","counters":{"domain_architectures":2266,"entries":34,"isoforms":0,"proteomes":1,"sets":3,"structures":0,"taxa":1,"dbEntries":{"smart":3,"profile":2,"cathgene3d":5,"ssf":2,"cdd":1,"pfam":3,"panther":1,"hamap":1,"ncbifam":1,"prints":1,"prosite":1,"interpro":13},"proteome":1,"taxonomy":1,"similar_proteins":2266}}}