{"metadata":{"accession":"F4H8R2","id":"F4H8R2_CELFA","source_organism":{"taxId":"590998","scientificName":"Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / CCUG 24087 / LMG 16345 / NBRC 15513 / NCIMB 8980 / NCTC 7547 / NRS-133)","fullName":"Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / CCUG 24087 / LMG 16345 / NBRC 15513 / NCIMB 8980 / NCTC 7547 / NRS-133)"},"name":"threonine ammonia-lyase","description":["Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA"],"length":400,"sequence":"MTGLAEVRAAARLLDGVAERTPVQLSRALGHVAGADVWLKCENLQRAGSFKIRGAYVRMSRLSPQERARGVVAASAGNHAQGVALAARMLGIDAVVYMPVDAALPKIAATRDYGARVELVGRTVDEALVHACEHAERTGAVLVHPFDHEDVVAGQGTVALEIVEQVPDVATVVVPLGGGGLAAGIVAALAEARPDVRVIGVQAARAAAYPASLQAHHPIPAPELRTMADGIAVGVPGTVPFEVLDRHHLEVRTVSEEDISRAVLLVAERAKLLVEPSGAVAVATIMATPGAWDGPVVALLSGGNIDPQVLLHVVRHGLASAGRFLQVRVRIDDKPGSLAALLRDLAATGGNVMHVSHVRTGSDLAFDQVTIQTQVETKGPEHCAQVLQHLRAAGYQVQPD","proteome":"UP000008460","gene":"Celf_2949","go_terms":[{"identifier":"GO:0004794","name":"threonine deaminase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006567","name":"L-threonine catabolic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"8bc361cd7c1d6142f8798dedc919bc5496ed8194","counters":{"domain_architectures":181949,"entries":16,"isoforms":0,"proteomes":1,"sets":3,"structures":0,"taxa":1,"dbEntries":{"cdd":2,"ssf":2,"cathgene3d":1,"profile":1,"panther":1,"pfam":1,"ncbifam":1,"interpro":7},"proteome":1,"taxonomy":1,"similar_proteins":181949}}}