{"metadata":{"accession":"E8N4K3","id":"E8N4K3_ANATU","source_organism":{"taxId":"926569","scientificName":"Anaerolinea thermophila (strain DSM 14523 / JCM 11388 / NBRC 100420 / UNI-1)","fullName":"Anaerolinea thermophila (strain DSM 14523 / JCM 11388 / NBRC 100420 / UNI-1)"},"name":"threonine ammonia-lyase","description":["Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA"],"length":313,"sequence":"MIFPWEWLEQAYQRLQDKVIQTPLTQDEVAAITLKWENQQITGSFKVRGALNRALTLQPWEIEKGLVCASAGNHGQGVAYAAKLLGASCIVFTYEGASAHKIEQMRQLGAKVHIVPGHYADAERAGLEYARSHQATWISPYNDPQVIAGQATVGLELIEQWKAEIPASVVVPVGGGGLAAGIALAMQRLPHPPKVIGVQSEASAYFHALFHQNNKKEVVEWESLADGLAGDVEDNSITIPLVKTHLFDLRLVSEKEIAEAIRFAWQRYGERIEGSAAVTLAAVLSRKVDELPATLIISGGNIDPQIFENILHS","proteome":"UP000008922","gene":"ANT_13350","go_terms":null,"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"8bc361cd7c1d6142f8798dedc919bc5496ed8194","counters":{"domain_architectures":181949,"entries":7,"isoforms":0,"proteomes":1,"sets":1,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cathgene3d":1,"panther":1,"pfam":1,"interpro":3},"proteome":1,"taxonomy":1,"similar_proteins":181949}}}