{"metadata":{"accession":"B6RJQ2","id":"B6RJQ2_9FALC","source_organism":{"taxId":"496007","scientificName":"Microhierax caerulescens caerulescens","fullName":"Microhierax caerulescens caerulescens"},"name":"V(D)J recombination-activating protein 1","description":["Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends"],"length":591,"sequence":"ILHGKYSQHLSSHKEMKDRGLYSHINKGGRPRQHLLSLTRRAQKHRLRELKRQVKAFAEKEEGGDIKAVCMTLFLLALRAKNEHRQADELEAIMQGRGSGLHPAVCLAIRVNTFLSCSQYHKMYRTVKAVTGRQIFQPLHALRTAEKALLPGYHPFEWKPPLKNVSTNTEVGIIDGLSGLPHSIDDYPVDTIAKRFRYDAALVCALKDMEEEILEGMKAKNVDDYLNGPFTVVVKESCDGMGDVSEKHGGGPAVPEKAVRFSFTVMNIAIAQGNESKRIFEEVKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKTSELLLEMGGILRTFKFIFRGTGYDEKLVREVEGLEASGSTYICTLCDATRLEASQNLVFHSITRSHAENLERYEIWRSNPYHESVDELRDRVKGVSAKPFIETVPSIDALHCDIGNATEFYRIFQMEIGEVYKNPDVSKEERKRWQLTLDKHLRKKMNLKPMMRMSGNFARKLMSKETVEAVCELIKCEERHEALKELMDLYLKMKPVWRSSCPAKECPELLCQYSYNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGS","proteome":null,"gene":"rag-1","go_terms":[{"identifier":"GO:0004519","name":"endonuclease activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0043565","name":"sequence-specific DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0061630","name":"ubiquitin protein ligase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0033151","name":"V(D)J recombination","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":true,"in_alphafold":true,"in_bfvd":false,"ida_accession":"b7cf38dce27f59e90f4eb2dce734de51b916b90c","counters":{"domain_architectures":109,"entries":20,"isoforms":0,"proteomes":0,"sets":2,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":2,"profile":2,"pfam":7,"panther":1,"interpro":8},"proteome":0,"taxonomy":1,"similar_proteins":109}}}