{"metadata":{"accession":"A0A6J2MPJ1","id":"A0A6J2MPJ1_9CHIR","source_organism":{"taxId":"89673","scientificName":"Phyllostomus discolor","fullName":"Phyllostomus discolor (pale spear-nosed bat)"},"name":"Adenylyltransferase and sulfurtransferase MOCS3","description":["Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions"],"length":458,"sequence":"MAAKAEVRALQAEVARREKELSSLKQRLAAALLAEEEPERPAPVSPLPPKAALTREEILRYSRQLVLPELGVQGQLRLTAASVLVVGCGGLGCPLAQYLAAAGVGRLGLVDYDVVEMSNLPRQVLHGEALAGQAKVFSAAASLRSLNSAVECVPYAQALTPATALDLVRRYDVVADCSDNAPTRYLVNDACVLAGRPLVSASALRFEGQITVYHYDGGPCYRCVFPQPPPADTVTNCADGGVLGVVTGVLGCLQALEVLKIAAGLGPSYSGQLMLFDALRGRFRCIRLRSRRPDCAACGEQPTVTDLQDYEAFCGSSATDKCRSLQLLSPEERVSVTDYKRLLDSGSPHVLLDVRPQVEVDICRLPHALHIPLKQLERRDAESLRLLGEAIQKGKRGTQEGAALPIYVICKLGNESQKAVKILQTLMAVQELGSLTVQDVVGGLMAWAAKIDGTFPQY","proteome":"UP000504628","gene":"MOCS3","go_terms":[{"identifier":"GO:0008641","name":"ubiquitin-like modifier activating enzyme activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016783","name":"sulfurtransferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0070566","name":"adenylyltransferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0002143","name":"tRNA wobble position uridine thiolation","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0005829","name":"cytosol","category":{"code":"C","name":"cellular_component"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"39f3ddf73e07cf4ac3a637cab78a1f5722c60329","counters":{"domain_architectures":10368,"entries":18,"isoforms":0,"proteomes":1,"sets":4,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cathgene3d":2,"cdd":2,"pfam":2,"smart":1,"profile":1,"hamap":1,"ncbifam":1,"panther":1,"interpro":6},"proteome":1,"taxonomy":1,"similar_proteins":10368}}}