{"metadata":{"accession":"A0A229WCD5","id":"A0A229WCD5_ASPFM","source_organism":{"taxId":"746128","scientificName":"Aspergillus fumigatus","fullName":"Aspergillus fumigatus"},"name":"Adenylyltransferase and sulfurtransferase uba4","description":["Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions","Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions"],"length":493,"sequence":"MENLEQTCASLRAQIAATEAQLAGLKRELEIAEQAAEVKAQSTTRTITAEDGKTNETREWPLLSEEYKRYGRQMIVPQLGLQGQLKLRAARVLIVGAGGLGCPAALYLAGAGVGTLGLVDGDTVENSNLHRQVLHRSKNVGTFKVDSAIEYLRELNPHPTYVPYRAHLTPQEAPGIFKDYDIVLDCTDNPATRYLISDTAVLLGKPLVSASALRTEGQLMVLNYPPRPVGDKSGGPCYRCVFPKPPPANSVVSCADGGILGPVVGTMGVLQALEAIKVITSPAVNPSASPPSLLIFSAYSTPLFRTIRLRARRANCAVCSADASVTLETLKNGSTDYVFFCGVAGLEATLSPEERISPLEFKKRHPKEVPQDGGRINKEPTIIDVREKVQFDICSLENSINIPISTILSSASSPTNVDANAQPSLPFWLPRELASADSTDPIYVVCRHGNDSQIAVRRLKELGLDRGGQRYVGDIQGGLRAWREQIDPDWPEY","proteome":null,"gene":"UBA4","go_terms":[{"identifier":"GO:0008641","name":"ubiquitin-like modifier activating enzyme activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016783","name":"sulfurtransferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0070566","name":"adenylyltransferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0002143","name":"tRNA wobble position uridine thiolation","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0005829","name":"cytosol","category":{"code":"C","name":"cellular_component"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"39f3ddf73e07cf4ac3a637cab78a1f5722c60329","counters":{"domain_architectures":10368,"entries":16,"isoforms":0,"proteomes":0,"sets":3,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cathgene3d":2,"cdd":1,"pfam":2,"smart":1,"profile":1,"hamap":1,"panther":1,"interpro":6},"proteome":0,"taxonomy":1,"similar_proteins":10368}}}