{"metadata":{"accession":"PF10435","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR018954","hierarchy":null,"name":{"name":"Beta-galactosidase, domain 2","short":"BetaGal_dom2"},"description":[{"text":"<p>This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique.  In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, [pfam:PF01301], which is N-terminal to it, but itself has no metazoan members.</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00031444":{"PMID":15491613,"ISBN":null,"volume":"343","issue":"5","year":2004,"title":"Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose.","URL":null,"raw_pages":"1281-92","medline_journal":"J Mol Biol","ISO_journal":"J. Mol. Biol.","authors":["Rojas AL","Nagem RA","Neustroev KN","Arand M","Adamska M","Eneyskaya EV","Kulminskaya AA","Garratt RC","Golubev AM","Polikarpov I."],"DOI_URL":"http://dx.doi.org/10.1016/j.jmb.2004.09.012"}},"set_info":{"accession":"CL0369","name":"GHD"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":114,"interactions":0,"matches":4386,"pathways":0,"proteins":4375,"proteomes":1577,"sets":1,"structural_models":{"alphafold":3467,"bfvd":0},"structures":15,"taxa":3177},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":116,"alignment:full":2779},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}