CENP-T/Histone H4, histone fold (IPR035425)

Short name: CENP-T/H4_C

Overlapping homologous superfamilies

Domain relationships



This domain is the C-terminal histone fold domain of CENP-T, which associates with chromatin [PMID: 21464230, PMID: 22304917]. Proteins containing this domain also include Histone H4.

CENP-T is a family of vertebral kinetochore proteins that associates directly with CENP-W. The N terminus of CENP-T proteins interacts directly with the Ndc80 complex in the outer kinetochore. Importantly, the CENP-T-W complex does not directly associate with CENP-A, but with histone H3 in the centromere region. CENP-T and -W form a hetero-tetramer with CENP-S and -X and bind to a ~100 bp region of nucleosome-free DNA forming a nucleosome-like structure. The DNA-CENP-T-W-S-X complex is likely to be associated with histone H3-containing nucleosomes rather than with CENP-nucleosomes [PMID: 22391098, PMID: 21464230, PMID: 22304917].

Histone H4 is one of the five histones, along with H1/H5, H2A, H2B and H3. Two copies of each of the H2A, H2B, H3, and H4 histones ensemble to form the core of the nucleosome [PMID: 16472024]. The nucleosome forms octameric structure that wraps DNA in a left-handed manner. H3 is a highly conserved protein of 135 amino acid residues [PMID: 8121801, PMID: 2041803]. Histones can undergo several different types of post-translational modifications that affect transcription, DNA repair, DNA replication and chromosomal stability. The sequence of histone H4 has remained almost invariant in more then 2 billion years of evolution [PMID: 8121801, PMID: 6808351, PMID: 3340182].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.