Endonuclease VIII (IPR023713)

Short name: Endonuclease-VIII

Overlapping homologous superfamilies

Family relationships



This entry represents the endonuclease 8 enzyme involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Endonuclease 8 acts as DNA glycosylase [PMID: 10862773] that recognises and removes damaged bases [PMID: 17002303]. The enzyme has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouraciland 5,6-dihydrothymine. It also has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand.The enzyme binds 1 zinc ion per subunit and acts by cleaving the DNA backbone by beta-delta elimination to generate a single-strand-break at the site of the removed base with both 3'- and 5'-phosphates. The C-O-P bond 3' to the apurinic orapyrimidinic site in DNA which is broken by the beta-elimination reaction leaves a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. This enzyme belongs to the FPG family and contains 1 FPG-type zinc finger [PMID: 16145054].

GO terms

Biological Process

GO:0006284 base-excision repair

Molecular Function

GO:0003684 damaged DNA binding
GO:0004519 endonuclease activity
GO:0008270 zinc ion binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.