Pathways & interactions
Serine carboxypeptidase, serine active site (IPR018202)
Short name: Ser_caboxypep_ser_AS
All known carboxypeptidases are either metallo carboxypeptidases or serine carboxypeptidases (EC:18.104.22.168 and EC:22.214.171.124). The catalytic activity of the serine carboxypeptidases, like that of the trypsin family serine proteases, is provided by a charge relay system involving an aspartic acid residue hydrogen-bonded to a histidine, which is itself hydrogen-bonded to a serine [PMID: 2324088]. Proteins known to be serine carboxypeptidases include:
- Barley and wheat serine carboxypeptidases I, II, and III [PMID: 2639682].
- Yeast carboxypeptidase Y (YSCY) (gene PRC1), a vacuolar protease involved in degrading small peptides.
- Yeast KEX1 protease, involved in killer toxin and alpha-factor precursor processing.
- Fission yeast sxa2, a probable carboxypeptidase involved in degrading or processing mating pheromones [PMID: 1549128].
- Penicillium janthinellum carboxypeptidase S1 [PMID: 8224168].
- Aspergullus niger carboxypeptidase pepF.
- Aspergullus satoi carboxypeptidase cpdS.
- Vertebrate protective protein / cathepsin A [PMID: 1907282], a lysosomal protein which is not only a carboxypeptidase but also essential for the activity of both beta-galactosidase and neuraminidase.
- Mosquito vitellogenic carboxypeptidase (VCP) [PMID: 1961751].
- Naegleria fowleri virulence-related protein Nf314 [PMID: 1587609].
- Yeast hypothetical protein YBR139w.
- Caenorhabditis elegans hypothetical proteins C08H9.1, F13D12.6, F32A5.3, F41C3.5 and K10B2.2.
This family also includes:
- Sorghum (s)-hydroxymandelonitrile lyase (EC 126.96.36.199) (hydroxynitrile lyase) (HNL) [PMID: 7948927], an enzyme involved in plant cyanogenesis.
The sequences surrounding the active site histidine residue are highly conserved in all these serine carboxypeptidases.
- PS00131 (CARBOXYPEPT_SER_SER)