Pathways & interactions
Peptidase M12A, nematode astacin 9/10/11 (IPR017368)
Short name: Peptidase_M12A_astacin-9/10/11
Overlapping homologous superfamilies
- Metallopeptidase, catalytic domain superfamily (IPR024079)
Over 70 metallopeptidase families have been identified to date. In these enzymes a divalent cation which is usually zinc, but may be cobalt, manganese or copper, activates the water molecule. The metal ion is held in place by amino acid ligands, usually three in number. In some families of co-catalytic metallopeptidases, two metal ions are observed in crystal structures ligated by five amino acids, with one amino acid ligating both metal ions. The known metal ligands are His, Glu, Asp or Lys. At least one other residue is required for catalysis, which may play an electrophillic role. Many metalloproteases contain an HEXXH motif, which has been shown in crystallographic studies to form part of the metal-binding site [PMID: 7674922]. The HEXXH motif is relatively common, but can be more stringently defined for metalloproteases as 'abXHEbbHbc', where 'a' is most often valine or threonine and forms part of the S1' subsite in thermolysin and neprilysin, 'b' is an uncharged residue, and 'c' a hydrophobic residue. Proline is never found in this site, possibly because it would break the helical structure adopted by this motif in metalloproteases [PMID: 7674922].
This family of metallopeptidases are found in Caenorhabditis elegans and belong to MEROPS peptidase family M12A (astacin, clan MA). They hydrolys peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
In the nematode C. elegans, 40 genes code for astacin-like proteins (nematode astacins, NAS). The astacins are metalloproteases present in bacteria, invertebrates and vertebrates and serve a variety of physiological functions like digestion, hatching, peptide processing, morphogenesis and pattern formation [PMID: 14653817].
This group represents a zinc metalloproteinase, nas-9/10/11 types.
- PIRSF038055 (Nas9/Nas10/Nas11)