Pyruvate formate-lyase 1 activating enzyme (IPR012838)

Short name: PFL1_activating

Overlapping homologous superfamilies

Family relationships


Pyruvate formate lyase-activating enzyme EC: is a 28 kDa monomeric protein which is involved in the anerobic glucose metabolism pathway. It is a member of the radical S-adenosylmethionine family of enzymes which initiate radical catalysis. It is necessary to activate pyruvate formate lyase (PFL), which catalyses the conversion of pyruvate and coenzyme A to formate and acetyl CoA.

Pyruvate formate lyase activating enzyme functions by catalysing the formation of the Gly734 radical of PFL. It utilises a [4Fe-4S] cluster and adenosylmethionine to generate a putative adenosyl radical, which extracts a hydrogen atom from Gly734 on PFL, thus activating PFL for catalysis [PMID: 15581584]. Members of this family are iron-sulphur proteins with a radical-SAM domain.

GO terms

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

GO:0043365 [formate-C-acetyltransferase]-activating enzyme activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.