Conserved Site

3-hydroxyacyl-CoA dehydrogenase, conserved site (IPR006180)

Short name: 3-OHacyl-CoA_DH_CS


3-hydroxyacyl-CoA dehydrogenase (EC: (HCDH) [PMID: 3479790] is an enzyme involved in fatty acid metabolism, it catalyzes the reduction of 3-hydroxyacyl-CoA to 3-oxoacyl-CoA. Most eukaryotic cells have 2 fatty-acid beta-oxidation systems, one located in mitochondria and the other in peroxisomes. In peroxisomes 3-hydroxyacyl-CoA dehydrogenase forms, with enoyl-CoA hydratase (ECH) and 3,2-trans-enoyl-CoA isomerase (ECI) a multifunctional enzyme where the N-terminal domain bears the hydratase/isomerase activities and the C-terminal domain the dehydrogenase activity. There are two mitochondrial enzymes: one which is monofunctional and the other which is, like its peroxisomal counterpart, multifunctional.

In Escherichia coli (gene fadB) and Pseudomonas fragi (gene faoA) HCDH is part of a multifunctional enzyme which also contains an ECH/ECI domain as well as a 3-hydroxybutyryl-CoA epimerase domain [PMID: 2204034].

The other proteins structurally related to HCDH are:

  • Bacterial 3-hydroxybutyryl-CoA dehydrogenase (EC: which reduces 3-hydroxybutanoyl-CoA to acetoacetyl-CoA [PMID: 8001771].
  • Eye lens protein lambda-crystallin [PMID: 3170592], which is specific to lagomorphes (such as rabbit).

There are two major region of similarities in the sequences of proteins of the HCDH family, the first one located in the N-terminal, corresponds to the NAD-binding site, the second one is located in the centre of the sequence.

GO terms

Biological Process

GO:0006631 fatty acid metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
GO:0016491 oxidoreductase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns