Methylglyoxal synthase (IPR004363)

Short name: Methylgl_synth

Overlapping homologous superfamilies

Family relationships



Methylglyoxal synthase (MGS) catalyzes the conversion of dihydroxyacetone phosphate (DHAP) to methylglyoxal and phosphate: Glycerone phosphate = methylglyoxal + phosphate

The first part of the catalytic mechanism is believed to be similar to TIM (triosephosphate isomerase) in that both enzymes utilize DHAP to form an ene-diolate phosphate intermediate. In MGS, the second catalytic step is characterized by the elimination of phosphate and collapse of the enediolate to form methylglyoxal instead of reprotonation to form the isomer glyceraldehyde 3-phosphate, as in TIM. This is the first reaction in the methylglyoxal bypass of the Embden-Myerhoff glycolytic pathway and is believed to provide physiological benefits under non-ideal growth conditions in bacteria [PMID: 10368300]. MGS is a small protein of about 13 to 17 kDa. An aspartate residue is involved in the catalytic mechanism [PMID: 10715115].

GO terms

Biological Process

GO:0019242 methylglyoxal biosynthetic process

Molecular Function

GO:0008929 methylglyoxal synthase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.