Active Site

Lipase, GDXG, putative histidine active site (IPR002168)

Short name: Lipase_GDXG_HIS_AS


The following lipolytic enzymes are evolutionary related:

  • Mammalian hormone sensitive lipase (HSL). In adipose tissue and heart, HSL primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
  • Mammalian arylacetamide deacetylase (DAC).
  • Moraxella strain TA144 lipase 2 (gene lip2), an enzyme active at a low temperature.
  • Acinetobacter calcoaceticus esterase which seems to be active on simple triglycerides such as triacetin.
  • Streptomyces hygroscopicus acetyl-hydrolase (gene bah), which removes the N-acetyl group from the antibiotic bialaphos.
  • Escherichia coli acetyl esterase.

These enzymes contain a Ser-centred consensus sequence and a conserved His-Gly dipeptide found in most lipase N-terminal domains. These sequences are involved in the lipase active site conformation since substitution of the conserved Ser or His residues by Ala and Gln, respectively, results in the loss of both lipase and esterase activities [PMID: 1907455].

This entry represents a conserved region containing the putative His active site.

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0016787 hydrolase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns