Glycoside hydrolase family 10 domain (IPR001000)

Short name: GH10

Overlapping homologous superfamilies

Domain relationships



O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PMID: 7624375, PMID: 8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Glycoside hydrolase family 10 GH10 comprises enzymes with a number of known activities; xylanase (EC:; endo-1,3-beta-xylanase (EC:; cellobiohydrolase (EC: These enzymes were formerly known as cellulase family F.

The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC:, cellobiohydrolases (EC: (exoglucanases), or xylanases (EC: [PMID: 2252383, PMID: 1886523]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family F [PMID: 2806912] or as the glycosyl hydrolases family 10 [PMID: 1747104].

The overall structure of the GH10 domain corresponds to an eightfold alpha/beta-barrel (TIM-barrel) with a typical deep groove in the centre, allowing an 'endo' type of action on the large polysaccharide backbone [PMID: 17642511], [PMID: 23508990].

GO terms

Biological Process

GO:0005975 carbohydrate metabolic process

Molecular Function

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles