{"metadata":{"accession":"P9WNU6","id":"PAFA_MYCTO","source_organism":{"taxId":"83331","scientificName":"Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)","fullName":"Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)"},"name":"Pup--protein ligase","description":["Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine. PafA is required to confer resistance against the lethal effects of reactive nitrogen intermediates (RNI), antimicrobial molecules produced by activated macrophages and other cell types"],"length":452,"sequence":"MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDSLVQLVTHDRAGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSETTTMLKVGTAALVLEMIESGVAFRDFSLDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQREYYTRAVEHLQTREPNAQIEQVVDLWGRQLDAVESQDFAKVDTEIDWVIKRKLFQRYQDRYDMELSHPKIAQLDLAYHDIKRGRGIFDLLQRKGLAARVTTDEEIAEAVDQPPQTTRARLRGEFISAAQEAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM","proteome":"UP000001020","gene":"pafA","go_terms":[{"identifier":"GO:0010498","name":"proteasomal protein catabolic process","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0019941","name":"modification-dependent protein catabolic process","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0016879","name":"ligase activity, forming carbon-nitrogen bonds","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"fb15860118f237b0124b05585d2f685e46d0a4a6","counters":{"domain_architectures":8673,"entries":7,"isoforms":0,"proteomes":1,"sets":1,"structures":0,"taxa":1,"dbEntries":{"panther":1,"hamap":1,"pirsf":1,"ncbifam":1,"pfam":1,"interpro":2},"proteome":1,"taxonomy":1,"similar_proteins":8673}}}