{"metadata":{"accession":"Q9M2P4","id":"SINA2_ARATH","source_organism":{"taxId":"3702","scientificName":"Arabidopsis thaliana","fullName":"Arabidopsis thaliana (Mouse-ear cress)"},"name":"E3 ubiquitin-protein ligase SINAT2","description":["E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:28351989, PubMed:32786047). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:28351989, PubMed:32786047). It probably triggers the ubiquitin-mediated degradation of different substrates (PubMed:28351989, PubMed:32786047). Mediates the proteasomal-dependent degradation of ATG6, a component of the autophagosome complex (PubMed:28351989). Requires TRAF1A/MUSE14 and TRAF1B/MUSE13 to target ATG6 for ubiquitination and subsequent regulation of autophagosome assembly (PubMed:28351989). Modulates directly the ubiquitination and proteasomal-dependent degradation of FREE1, a component of the ESCRT-I complex (PubMed:32753431, PubMed:32786047). Modulates directly the ubiquitination and proteasomal-dependent degradation of ELC/VPS23A, a component of the ESCRT-I complex (PubMed:32753431)"],"length":308,"sequence":"MAPGGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE","proteome":"UP000006548","gene":"SINAT2","go_terms":[{"identifier":"GO:0008270","name":"zinc ion binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005515","name":"protein binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006511","name":"ubiquitin-dependent protein catabolic process","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0007275","name":"multicellular organism development","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0005737","name":"cytoplasm","category":{"code":"C","name":"cellular_component"}}],"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"3f468851f694fe28c25bcae276188b81757b837b","counters":{"domain_architectures":5800,"entries":19,"isoforms":2,"proteomes":1,"sets":5,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":2,"ssf":2,"cdd":2,"pfam":3,"profile":2,"panther":1,"interpro":7},"proteome":1,"taxonomy":1,"similar_proteins":5800}}}