{"metadata":{"accession":"Q58597","id":"SYT_METJA","source_organism":{"taxId":"243232","scientificName":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","fullName":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)"},"name":"Threonine--tRNA ligase","description":["Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed:15240874). Also activates L-serine, but does not detectably transfer it to tRNA(Thr) (PubMed:15240874). Edits incorrectly charged L-seryl-tRNA(Thr) via its editing domain (PubMed:15240874). Has no activity on correctly acylated L-seryl-tRNA(Ser) or L-threonyl-tRNA(Thr) (PubMed:15240874). Deacylates correctly charged glycyl-tRNA(Gly), but not glycyl-tRNA(Gly)(2'-dA76) (the terminal 2'-OH of tRNA adenine 76 has been dehydroxylated) nor the 2'-fluoro tRNA derivative, strongly suggesting the editing function is tRNA catalyzed (PubMed:26113036)"],"length":620,"sequence":"MKMLLIHSDYLEFEAKEKTKIAEETENLKGKLDECLACFIAVEREDENNPEGTAIGAVEEIEKVANQLKVNNIVVYPYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGWYKAFKISCKGHPLSELSRKIVAKEEKKEEGEESKFYLLNPETEEIIELNENNINIIKDEELLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKLMRDLLADYVYNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFAACFGQFMMKKDMYLLPRYLPLKLYELSTYSFRYEQRGELVGLKRLRCFTMPDMHTVCLNLEQAMEEFEKQFWECLKTGDDLNLSYSVIFRFTKDFFDEHRDWFFKIAKEYKNKYGKDVILEILPKRKHYWVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEGEIYPIILHCSPTGSIERVLCGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEKLRENNIRADFDDREESVSKKIRNAGKEWVPYVVVIGDEEMESDKLTVTIREKSTLKKPYKEKMTLDELIERIKKETANYPYRPLPLPIRCSLQPKFH","proteome":"UP000000805","gene":"thrS","go_terms":[{"identifier":"GO:0004829","name":"threonine-tRNA ligase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005524","name":"ATP binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0008270","name":"zinc ion binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005737","name":"cytoplasm","category":{"code":"C","name":"cellular_component"}},{"identifier":"GO:0000166","name":"nucleotide binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0004812","name":"aminoacyl-tRNA ligase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006418","name":"tRNA aminoacylation for protein translation","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0006435","name":"threonyl-tRNA aminoacylation","category":{"code":"P","name":"biological_process"}}],"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"23e3e9e0d32fd37fe7b0ef10228a8ffd9d7b2bcb","counters":{"domain_architectures":484,"entries":26,"isoforms":0,"proteomes":1,"sets":4,"structures":2,"taxa":1,"dbEntries":{"cathgene3d":3,"pfam":3,"ssf":2,"cdd":2,"profile":1,"ncbifam":2,"hamap":1,"panther":1,"prints":1,"interpro":10},"proteome":1,"taxonomy":1,"similar_proteins":484}}}