{"metadata":{"accession":"Q4N6U8","id":"Q4N6U8_THEPA","source_organism":{"taxId":"5875","scientificName":"Theileria parva","fullName":"Theileria parva (East coast fever infection agent)"},"name":"Queuine tRNA-ribosyltransferase catalytic subunit 1","description":["Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product"],"length":407,"sequence":"MALKFNVVARDGRARRGELILPHGVVETPVFMPVGTKGAIKGVCYDDLESLGCVLSLGNTFHLAFHPGSEIIRKSGGLHKFSNSRCNFLTDSGGFQMVSLFSLSKVTEEGVEFKFPYDLEREIFLTPEESIKIQNDIGADIIMALDDVVSSIVRDKERLELATHRTTRWLDRCIKAHSNPETQALFAIVQGSIYPDLRRISLNELKKRDLPGYAIGGLSGGENKTDFWRIVELCTREEIGLPFNKPRYLMGVGYQIDLIVSVALGCDMFDCVFPTRTARFGTALNDFGNIRIQHEIYQYDFSPIDQDCDCVCCRKYSRAFLHYGLKKSILVSHLLTLHNIRYMVRLFEQIRESISEGNFKGFVRRFLTNMYINNGELPPLWVRDSLHSAGIDILDLYPDNSDVVKPY","proteome":"UP000001949","gene":"TP01_1072","go_terms":[{"identifier":"GO:0006400","name":"tRNA modification","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0008479","name":"tRNA-guanosine(34) queuine transglycosylase activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"eafd7fbc2e41b85ce18cb590f0612d798045897d","counters":{"domain_architectures":31634,"entries":10,"isoforms":0,"proteomes":1,"sets":1,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cathgene3d":1,"pfam":1,"panther":1,"hamap":1,"ncbifam":2,"interpro":3},"proteome":1,"taxonomy":1,"similar_proteins":31634}}}