{"metadata":{"accession":"Q3S2U2","id":"MOKF_MONPI","source_organism":{"taxId":"89488","scientificName":"Monascus pilosus","fullName":"Monascus pilosus (Red mold)"},"name":"Acyltransferase mokF","description":["Acyltransferase; part of the gene cluster that mediates the biosynthesis of monakolin K, also known as lovastatin, and which acts as a potent competitive inhibitor of HMG-CoA reductase (PubMed:18578535, PubMed:19693441). MokF catalyzes the last step of the pathway by adding the side-chain (2R)-2-methylbutanoate produced by the diketide synthase mokB onto monacoline J to yield monacoline K (By similarity). Monakolin K biosynthesis is performed in two stages. The first stage is catalyzed by the nonaketide synthase mokA with the help of the enoyl reductase mokE that catalyze the iterative nine-step formation that leads to the formation of dihydromonacolin L. Covalently bound dihydromonacolin L is released from mokA by the mokD esterase. Conversion of dihydromonacolin L into monacolin L and then monacolin J is subsequently performed with the participation of molecular oxygen and P450 monoogygenase mokC. Finally, mokF performs the conversion of monacoline J to monacoline K through the addition of the side-chain diketide moiety (2R)-2-methylbutanoate produced by the diketide synthase mokB (Probable)"],"length":413,"sequence":"MRQFLSSDRINSEIPQEKSEMVGFSDIDNSSRQIKEMEAAFRSAVKTGQIPGAVIMARDHSGRLNYTRCFGARTVVRDECNRLPPMQVDTPCRLASATKLLTTIMALQCVERGLVRLDETVDRLLPDLSAMKVLEGFDAAGEPKMRERKGKITLKHLLTHTSGLSYVFLHPLLREYMAKGHLQTAEKFGIQSRLAPPAVNDPGAEWIYGANLDWTGKLVERATGLDLEQYLQENICAPLNITDMTFKLQQRPDLLARRADQTHRNKADGRLRYDDSVYFRSDGDECFGGQGVFSGPESYMKVVHSLLQRDGRLLRPETVDLMFQPALDAQTEKQMNQHMDASPHINYGGPMPMVLRRSFGLGGMIALEDLDGQKWRRKGCLTFGGGPNIVWVMLLSALRFVFFFFFFFFFCSS","proteome":null,"gene":"mokF","go_terms":null,"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"6eccaef758bed472747734f6b0565810a6ecc3b1","counters":{"domain_architectures":128765,"entries":7,"isoforms":0,"proteomes":0,"sets":1,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"ssf":1,"pfam":1,"panther":1,"interpro":3},"proteome":0,"taxonomy":1,"similar_proteins":128765}}}