{"metadata":{"accession":"Q161G5","id":"MIAB_ROSDO","source_organism":{"taxId":"375451","scientificName":"Roseobacter denitrificans (strain ATCC 33942 / OCh 114)","fullName":"Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114))"},"name":"tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase","description":["Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine"],"length":441,"sequence":"MTAPKKLFIKTYGCQMNVYDSERMSEALVAEGYVETKTAEDADMILLNTCHIREKAAEKVYSELGRLKSLKADNPDLKLGVAGCVAQAEGEEIMRRQPAVDLVVGPQSYHRLPQMEARLREGHKALDTDFPPEDKFEELKARPKARRAPSAFLTVQEGCDKFCAFCVVPYTRGAEVSRPVTRVLDEARDLVERGVREITLLGQNVNAYHGAGADGNEKTLAQLIWALNDIDGLERIRFTTSHPNDMQDDLIEAHRDCPKLMPYLHLPVQSGSDRILKRMNRKHTADSYLRLIERIRVGRPDILLSGDFIVGFPEETEADFQATLDLIEAVNYGYAYSFKYSTRPGTPAAERAQVDPVEADERLQRIQALITRQQQDIQQSMVGRDVSVLIEKPGRFEGQMVGKSEYLHAVHVDQCSAQIGDILPVRIVEAKRNSLAAVTLA","proteome":"UP000007029","gene":"miaB","go_terms":[{"identifier":"GO:0035596","name":"methylthiotransferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0051539","name":"4 iron, 4 sulfur cluster binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003824","name":"catalytic activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0051536","name":"iron-sulfur cluster binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016740","name":"transferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006400","name":"tRNA modification","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"174dc51e9bfbb664d85de61124045069ab770994","counters":{"domain_architectures":21985,"entries":30,"isoforms":0,"proteomes":1,"sets":4,"structures":0,"taxa":1,"dbEntries":{"profile":3,"cathgene3d":2,"ssf":1,"smart":1,"pfam":3,"cdd":1,"sfld":4,"hamap":1,"panther":1,"ncbifam":2,"prosite":1,"interpro":10},"proteome":1,"taxonomy":1,"similar_proteins":21985}}}