{"metadata":{"accession":"M1T7M1","id":"M1T7M1_9VIRU","source_organism":{"taxId":"262588","scientificName":"Cardamom bushy dwarf virus","fullName":"Cardamom bushy dwarf virus"},"name":"Master replication protein","description":["Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities"],"length":286,"sequence":"MARYVVCWMFTINNPSSLPVMRDEIKYMVYQVERGQEGTRHVQGYVEMKRRSSLKQMRMFFPGAHLEKRKGTQEEARTYCMKEDTRIEGPFEFGVFKLACNDSLYDVIQDMRLTNKRPLEYLYDCPNTFDRSKDTLYRVQAEMNKTKAMESWGNSYGSWTEQVEELMAQPCHRRIIWVYGPNGGEGKTTYAKHLMKTRNAFYTPGGKALDICRLYNYEEIVIFDIPRCKEEYINYGIIEEFKNGIVQSGKYEPVLKIVEYVEVIVMANFLPKEGIFSEDRIKVVAC","proteome":null,"gene":"Rep","go_terms":[{"identifier":"GO:0006260","name":"DNA replication","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0003723","name":"RNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003724","name":"RNA helicase activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":false,"in_bfvd":false,"ida_accession":"cb80c3b031ca2fd79bbbd2adcc26ed7bfeea18ca","counters":{"domain_architectures":4697,"entries":6,"isoforms":0,"proteomes":0,"sets":2,"structures":0,"taxa":1,"dbEntries":{"profile":1,"cathgene3d":1,"pfam":2,"interpro":2},"proteome":0,"taxonomy":1,"similar_proteins":4697}}}