{"metadata":{"accession":"M0MUH6","id":"M0MUH6_HALMO","source_organism":{"taxId":"931277","scientificName":"Halococcus morrhuae DSM 1307","fullName":"Halococcus morrhuae DSM 1307"},"name":"(S)-8-amino-7-oxononanoate synthase BioU","description":["A 'suicide' enzyme that participates in biotin synthesis. Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD. The cellular requirement for biotin is thought be low enough that this single turnover enzyme supplies a sufficient amount of the cofactor. Overall it catalyzes three reactions: formation of a covalent linkage with 8-amino-7-oxononanoate to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)+"],"length":341,"sequence":"MHDIRFAVLGTGGIGRRALDVSREKPGLLPVAACDRNGIAIDHDGLDVDELLAATEGNVASDGGAAVKQHGAGAGVVASAQARSTETPIEDIIAESDAIDAVLIALPNLEHDFIPRVAERFTGAGYEGVLVDVLKRSRVIGMLDGRADSFEESGITFVCGAGATPGFLTGAAALAAQSFVEIESVEIHWGVGLKSGYEDNRGTVREDIAHLPGYDIETARELTDEEIEGIVDEHDGVIEFEEMEHADDVLLERAGICDADDVSVGGILDVRSDEKPTTTTVSVTGRTFDGEHATNTFELGDETSMAANVNGPALGYLKAGVQRNRAGEYGVFGPADLMPEF","proteome":"UP000011568","gene":"bioU","go_terms":[{"identifier":"GO:0008483","name":"transaminase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0009102","name":"biotin biosynthetic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":null,"counters":{"domain_architectures":0,"entries":5,"isoforms":0,"proteomes":1,"sets":0,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"ssf":1,"hamap":1,"interpro":2},"proteome":1,"taxonomy":1}}}