{"metadata":{"accession":"K3VJJ1","id":"FCK3_FUSPC","source_organism":{"taxId":"1028729","scientificName":"Fusarium pseudograminearum (strain CS3096)","fullName":"Fusarium pseudograminearum (strain CS3096) (Wheat and barley crown-rot fungus)"},"name":"Probable glycosyltransferase FCK3","description":["Probable glycosyl transferase; part of the gene cluster that mediates the biosynthesis of cytokinins such as fusatin, fusatinic acids or 8-oxofusatin, known for their growth promoting and anti-senescence activities toward host plants (PubMed:28802024). FCK1 is a bifunctional enzyme that performs the first steps in the biosynthesis of Fusarium cytokinins (PubMed:28802024). It first condenses adenosine monophosphate (AMP) with dimethylallyl diphosphate (DMAPP) to yield isoprenyl adenosine monophosphate (PubMed:28802024). It then catalyzes the removal of the phosphoribose to produce isopentenylaldehyde (PubMed:28802024). The cytochrome P450 monooxygenase then converts isopentenylaldehyde to trans-zeatin (PubMed:28802024). A condensation step converts trans-zeatin to fusatin which is further modified to produce fusatinic acid (PubMed:28802024). The mechanism for oxidation of fusatin to fusatinic acid remains unknown (PubMed:28802024). 8-oxofusatin could be produced through several pathways, via direct oxygenation of fusatin, or via the 8-oxo-pentenyladenine intermediate which itself must arise from either the prenylation of 8-oxo-AMP by FCK1 and/or oxygenation of isopentenylaldehyde (PubMed:28802024). Both the FCK3 and FCK4 enzymes act downstream of the identified cytokinins to produce yet unidentified compounds (PubMed:28802024)"],"length":394,"sequence":"MHFAIPLEYQSELEATEPVDVGTDEEIISSIEQYRPVTSEKNIWAFWDSGILSMPSWCKRNVIGWARICGADWTIRVLDMKPNSPNHVLKFIDRDMLPEAFLSGTMDGHHTGQHSADFIRGPLLHHYGGVSMDVGCLLIRHIDRICWDLLADPDSPYEIAVPVLYDQTIANHFIAARKNNIFIEKWHQLFLHLWNGRTHQQGISDSPLLGFIKDIRYDDATDFHWDWSVPVPQFLEYIAQVLCWQRLCLIRDTGDGFKSSEYWQRNVLCIDSLNEVWGGEKTLGFDGIGPRMYNLLTTRLDADPDSTAYKDAYKLVWRLLTRSSFQKVTRAKNLTYTPHLGTLWDQNEGKDCIPGSFGELLRYGPVHFRQKRENIEQLEASEPRTLIEKGLLEV","proteome":"UP000007978","gene":"FCK3","go_terms":[{"identifier":"GO:0016757","name":"glycosyltransferase activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":2,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"a31fa28f47baafa628093a73fe1ddda3e5efbc80","counters":{"domain_architectures":2794,"entries":5,"isoforms":0,"proteomes":1,"sets":1,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"ssf":1,"pfam":1,"interpro":2},"proteome":1,"taxonomy":1,"similar_proteins":2794}}}