{"metadata":{"accession":"G9CM47","id":"G9CM47_9GEMI","source_organism":{"taxId":"10831","scientificName":"Tomato golden mosaic virus","fullName":"Tomato golden mosaic virus"},"name":"Replication-associated protein","description":["Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities"],"length":352,"sequence":"MPSHPKRFQINAKNYFLTYPQCSLSKEESLSQLQALNTPINKKFIKICRELHEDGQPHLHVLIQFEGKYCCQNQRFFDLVSPTRSAHFHPNIQRAKSSSDVKTYIDKDGDTLVWGEFQVDGRSARGGCQTSNDAAAEALNASSKEEALQIIREKIPEKYLFQFHNLNSNLDRIFDKTPEPWLPPFHVSSFTNVPDEMRQWAENYFGKSSAARPERPISIIIEGDSRTGKTMWARSLGPHNYLSGHLDLNSRVYSNKVEYNVIDDVTPQYLKLKHWKELIGAQRDWQTNCKYGKPVQIKGGIPSIVLCNPGEGASYKVFLDKEENTPLKNWTFHNAKFVFLNSPLYQSSTQSS","proteome":null,"gene":"AC1","go_terms":[{"identifier":"GO:0006260","name":"DNA replication","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0016888","name":"DNA endonuclease activity, producing 5'-phosphomonoesters","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005198","name":"structural molecule activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":false,"in_bfvd":false,"ida_accession":"3576995e52844acc3074eed64c2fbc93b5358a47","counters":{"domain_architectures":9115,"entries":11,"isoforms":0,"proteomes":0,"sets":1,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cathgene3d":1,"pfam":2,"profile":1,"prints":2,"interpro":4},"proteome":0,"taxonomy":1,"similar_proteins":9115}}}