{"metadata":{"accession":"F8AN23","id":"F8AN23_METOI","source_organism":{"taxId":"647113","scientificName":"Methanothermococcus okinawensis (strain DSM 14208 / JCM 11175 / IH1)","fullName":"Methanothermococcus okinawensis (strain DSM 14208 / JCM 11175 / IH1)"},"name":"CCA-adding enzyme","description":["Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded"],"length":451,"sequence":"MDKENFELEDVKEVLDGLLNDISPSKEEETKIKEFSKKIINELYDELNNEKLRHLIVDIVQVGSTARNTNLKNDYDIDIFIRFKKGVDKEVLKETVLKIGKKVIEKLNGKYWIEYAEHPYISAKIGRYDIDIVPCYKLNWGENIVSAVDRTPLHNEFLKKMLKSNTLNNEVRLLKKFLKGIGIYGSDLKTKGFSGYLCELLIIHYGGFINTLIGAQKWKLGKKIILNEIYNIYKLNKDYKFADFKDPLVVYDPVDLNRNVAAALSKENFCKFIFYSYQFLKNPSKDFFYDYHKNLNEKLGNRKKGMLFTLGIRRNKDIVDDVIYPQMEKLQKSINKILIENDFEFLRCGNYADDDMCYLSWEFLVWELPNIKLKIGPPVFNKKGVDNFIKKNDKYFVKDCNVCAYVERNYKNVDELFEAIAEGKLKKKISYPKYVSPENAVIFKGIYKEYE","proteome":"UP000009296","gene":"cca","go_terms":[{"identifier":"GO:0016779","name":"nucleotidyltransferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0004810","name":"CCA tRNA nucleotidyltransferase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003723","name":"RNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0031123","name":"RNA 3'-end processing","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0001680","name":"tRNA 3'-terminal CCA addition","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"07b3e75f19cb0ade4595b4daf36179ecc847876f","counters":{"domain_architectures":880,"entries":23,"isoforms":0,"proteomes":1,"sets":2,"structures":0,"taxa":1,"dbEntries":{"ssf":3,"cathgene3d":4,"cdd":1,"pfam":3,"hamap":1,"panther":1,"ncbifam":1,"pirsf":1,"interpro":8},"proteome":1,"taxonomy":1,"similar_proteins":880}}}