{"metadata":{"accession":"F6KX03","id":"F6KX03_GOWLO","source_organism":{"taxId":"118216","scientificName":"Gowidon longirostris","fullName":"Gowidon longirostris (Long-nosed water dragon)"},"name":"V(D)J recombination-activating protein 1","description":["Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends"],"length":459,"sequence":"VAATLDKVTENQTEVSTIPSEVCSETDTELNKSIQTTNKDTFHLNQKETENHQTNLQKLCRICGGSFKSDPYKRSYPVHGPVDDDTQALLRKKEKRATSWPDLLAKVFKIDVRGDIDTIHPTNFCHNCWNVIQRKFSNSPSEVYFPRKGTMEWHPHSSSCDVCGTSFHGIKRKKRSLNPQLSKKPRIVAGSTRKVRPVRNVKQVSNKAVMKRIANCNKIHLSTKILAVDYPVDFVKSVSCQVCEHILADPVETTCKHLFCRACILKCIKAMGSYCPACRYPCFPTDLVSPVKSFLNILNNLILRCPVKDCQEEVVLGKYAHHFSSHKEAEEKEGYAYINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFAEKEEGGDVKSVCLTLFLLALRARNEHRQADELEAIMQGKGSGLHPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRTAE","proteome":null,"gene":"RAG1","go_terms":[{"identifier":"GO:0046872","name":"metal ion binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0004519","name":"endonuclease activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0043565","name":"sequence-specific DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0061630","name":"ubiquitin protein ligase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0033151","name":"V(D)J recombination","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":true,"in_alphafold":true,"in_bfvd":false,"ida_accession":"4a9c7dc7f79f264b030eaa2578b48905938c548c","counters":{"domain_architectures":749,"entries":28,"isoforms":0,"proteomes":0,"sets":3,"structures":0,"taxa":1,"dbEntries":{"ssf":2,"cathgene3d":3,"cdd":1,"smart":1,"pfam":5,"profile":3,"panther":1,"prosite":1,"interpro":11},"proteome":0,"taxonomy":1,"similar_proteins":749}}}