{"metadata":{"accession":"F0LL89","id":"F0LL89_THEBM","source_organism":{"taxId":"391623","scientificName":"Thermococcus barophilus (strain DSM 11836 / MP)","fullName":"Thermococcus barophilus (strain DSM 11836 / MP)"},"name":"Reverse gyrase","description":["Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection","Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection"],"length":1216,"sequence":"MKAIYREMCPNCEDRISDERLYKKHPCDVCLDREIQAEIYFDLITGIREALKLRRTLKHWENIYKLEKKLREIEDLFEKATGFNFWSAQKTWVKRLIKNKSFSIIAPTGMGKSVFGSFMALYYAKKKKKSYIVLPTTPLVVQTIKRVKTFAERAGIEINLAYYHGGMKKKEKEEMTEKIMSGDFDILITSAQWLARNFDEMLKDKRFDFIFVDDVDAFLKASKNIDRSLILLGFTEEIIQKAWEIIRLKKQMAKFLNGNSPDKQEQLKELNKQINKLEREIRKFKRENKIGVLIVASATGSARGDRIKLYRELLDFEVGSGRSALRNVVDSYLFPEKSIEEHVEELLTKLGKGGLIFVPIDQGISYAEKLTQYLQEKGFKVELVSARNKKGLEKFENEEIDYLVGVATYYGTIVRGLDLPHLIRYAIFTGVPKFRFSLDLEQPTIYRVLGLMSEVMEFLDEEDRREGERLYARLRRLIRNIPQFELMKIEEALAEGLPLEGFHSHVLEVFKQSVEFLRRVLKKEEVLKRIEEDPFVSLKEEEGKWYIEIPDVRTYIQASGRTSRLFAGGITKGLSVIIVDNEKVFNGLKRQMRWRFAEFEMKPFEELNLEELLREIDSDREKVRLILAGKISEKTKDLVKSALMIVESPNKARTIANFFGQPSKRRIGDLVAYEVSIGDRMLTILASGGHMFDLVTTEGYHGVLIEERDGKLYFIPVYDTIKRCRDCGHQFVDWEERGVCPRCGSRNVRDALENVIAMREIAQEVDEILIGTDPDVEGEKIAWDIRNVLSPYTPNIKRIEFHEVTRPAILKALNEARDVNEFRVNAQLVRRIEDRWIGFELSQKLWEVFESRNLSAGRVQTPVLGWVIQRYKEFVESETDFMALTLENGLSVTLEGVKDEVNEIIVEDVQIEEKEINPFPPYTTDTMLQDASRFLGFSAPHTMQLAQDLFELGLITYHRTDSTHVSNTGIEVAKEYISEEIGEEYFAPRKWGEEGAHECIRPTRPIDTGRLMQLLRDGIITLARGLTRDHFRLYDMIFKRFMASQMKAAKVLYEKAVIDARIAKTEIEGYVEIIFDGWTKLRAPPLKQLPRLEKGQKLKVIEVKKWRAPKVSLYTQGDLIALMKERKIGRPSTYAKIVKTLLDRHYVIETKGRKKLVPTELGTKVYHYLISKYKELVSEERTRQLEELMDLVEENKADYQEVLSNMYEEIKQYILK","proteome":"UP000007478","gene":"rgy","go_terms":[{"identifier":"GO:0003676","name":"nucleic acid binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0005524","name":"ATP binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003677","name":"DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003916","name":"DNA topoisomerase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006265","name":"DNA topological change","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"3a26e0e3356a7336345ac099af0e7b55d795dd85","counters":{"domain_architectures":336,"entries":41,"isoforms":0,"proteomes":1,"sets":7,"structures":0,"taxa":1,"dbEntries":{"profile":5,"pfam":4,"ssf":2,"cathgene3d":5,"cdd":4,"smart":4,"hamap":1,"panther":1,"ncbifam":1,"prints":1,"interpro":13},"proteome":1,"taxonomy":1,"similar_proteins":336}}}