{"metadata":{"accession":"E8T6Y3","id":"E8T6Y3_THEA1","source_organism":{"taxId":"648996","scientificName":"Thermovibrio ammonificans (strain DSM 15698 / JCM 12110 / HB-1)","fullName":"Thermovibrio ammonificans (strain DSM 15698 / JCM 12110 / HB-1)"},"name":"DNA topoisomerase 1","description":["Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone"],"length":648,"sequence":"MLVIVESPTKAKKIQSFLGKGYTVKASLGHVRDLPPDEFAVSIEALEQGKFPFKFKVLAGKGRVVKELQRLASGELVLCASDPDREGEAIAWHLAQLLKEKAKEVKRIEFHEITKKALQEAVKRPREIDLNRVRAQFARRAIDRILGYLISPELSKELGKRGLSAGRVQSTALAEIVKREREIKEFVPKPYWVVEVELEKDGKKFRARTEKFWDKKEAQENLRFPTLLVIRADKRKKLEKPKAPFTASALQQEANRLFKWSPEYTMKVAQELFENGYITYHRSDSPRLSEEAVGFLRNLIPRLFGKDYLPAKPFVYKAKEGAQDAHEAIRPTKLTPEGAPENLKDRLTPQQLKLYTLIWKRTLASQMKEAVWNTQTVVLQNDRGVKFTAKGKTLVFEGWRKVYDAEVEEDGEGILPELRVGEKLPAKLRLKEDKTKPPARYTEGSLVKWLEKTGVGRPSTYASTVKTLKARKYVVVKGGKMVPTETAFRVIEFLEEKHPWILSPELTAQMEKQLDLVEQGKLDWRKPVLETVEKVRELLPLLSEERDDRPTEKQLEFARSLAEKLKKELPADVLLSKKKLSKWIDGAKRELAKEKANAPLSEKQVAVIEKHGDEKVREALLRGDYAYCRKWLDGFFRELKRSGRRKKK","proteome":"UP000006362","gene":"topA","go_terms":[{"identifier":"GO:0003677","name":"DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003916","name":"DNA topoisomerase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006265","name":"DNA topological change","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0003917","name":"DNA topoisomerase type I (single strand cut, ATP-independent) activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"50b5241a90332a8fc9fc2d4a42095c76d99ebd7a","counters":{"domain_architectures":15769,"entries":30,"isoforms":0,"proteomes":1,"sets":3,"structures":0,"taxa":1,"dbEntries":{"profile":2,"cathgene3d":4,"smart":3,"cdd":2,"ssf":1,"pfam":2,"ncbifam":1,"panther":1,"hamap":1,"prints":1,"interpro":12},"proteome":1,"taxonomy":1,"similar_proteins":15769}}}