{"metadata":{"accession":"D3H3Y2","id":"D3H3Y2_ECO44","source_organism":{"taxId":"216592","scientificName":"Escherichia coli O44:H18 (strain 042 / EAEC)","fullName":"Escherichia coli O44:H18 (strain 042 / EAEC)"},"name":"L-threonine dehydratase","description":["Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA"],"length":514,"sequence":"MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIAQNNIRGERLAHILSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRFLNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAFRFFLAG","proteome":null,"gene":"ilvA","go_terms":[{"identifier":"GO:0004794","name":"threonine deaminase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0009097","name":"isoleucine biosynthetic process","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0030170","name":"pyridoxal phosphate binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006520","name":"amino acid metabolic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"dca8c35a0c02c1a2752d0ea9189b163faeded61c","counters":{"domain_architectures":9770,"entries":22,"isoforms":0,"proteomes":0,"sets":4,"structures":0,"taxa":1,"dbEntries":{"ssf":2,"cdd":3,"cathgene3d":2,"pfam":2,"profile":1,"ncbifam":2,"panther":1,"prosite":1,"interpro":8},"proteome":0,"taxonomy":1,"similar_proteins":9770}}}