{"metadata":{"accession":"C4R208","id":"C4R208_KOMPG","source_organism":{"taxId":"644223","scientificName":"Komagataella phaffii (strain GS115 / ATCC 20864)","fullName":"Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)"},"name":"Cytoplasmic tRNA 2-thiolation protein 2","description":["Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation"],"length":457,"sequence":"MAFTDSDATSQRISTSQNICQRCKNMGSICFSRKEYFCKDCFLRFIRGKQRKLMMEDKFKVQYPQKGVESVSQNVMLALSMGSSSLVLFDILFNLLLEQTKTHRGRQGFHLKVVIVDESTLSDSDYSVRDKVEELKEAYGIDHNCPVDVSFKIVDPNQFILDSDQVGSILIDNDFQSYITGNNSEDTKKLTITEILDQTPNRSSREDILEMVIYQLLIRTCLVINCQTILFGHSMTKLADQVISLTVRGRASLIGKRLTDAVEIHNGSEIHIIHPLRDVLKSEVVHYSEMLQLERFNVMNQKKAADLLKSKVNKSMTVNELTRSYFDSIEGDYSEVVSTVVKIGTKLDQLRSTEHNKRCIVCDIQLSSDPKHWLENITVNEPAPVVTEADINNLRIYSETCLLEDDASKLVMNPGSEVDMCYACLTSLQDIRSAFRWPVARASKEQILDEFTIGDDI","proteome":"UP000000314","gene":"NCS2","go_terms":[{"identifier":"GO:0000049","name":"tRNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0002098","name":"tRNA wobble uridine modification","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0034227","name":"tRNA thio-modification","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"bd1c1e17b4fee948dbf7b791cb65367063e040b0","counters":{"domain_architectures":3300,"entries":7,"isoforms":0,"proteomes":1,"sets":0,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"ssf":1,"hamap":1,"panther":1,"pfam":1,"interpro":2},"proteome":1,"taxonomy":1,"similar_proteins":3300}}}