{"metadata":{"accession":"A0AB36TTV5","id":"A0AB36TTV5_BACTU","source_organism":{"taxId":"1428","scientificName":"Bacillus thuringiensis","fullName":"Bacillus thuringiensis"},"name":"DNA topoisomerase 3","description":["Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone"],"length":729,"sequence":"MSKSVVIAEKPSVARDIARVLKCDKKGNGYLEGNKYIVTWALGHLVTLADPESYDVKYKKWNLEDLPMLPERLKLTVIKQTGKQFNAVKSQLLRKDVNEIIVATDAGREGELVARWIIDKVKLNKPIKRLWISSVTDKAIKDGFANLKPGKAYDNLYASAVARSEADWYIGLNATRALTTRFNAQLNCGRVQTPTVAMIASREDEIKNFKAQTYYGIEAQTMEKLKLTWQDTNGNSRSFNKEKIDGIVKSLDKQNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISSDIVGTLPERLKACGVGEYRPFAHKVLQKPIKPNKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFPAFEYEQLTLRTKVGSETFIARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPHIEKGDTLTVKLLMQTSGQTKAPARFNEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPTLTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGHRKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQNKEEEPLNNPFAEALKKLKFD","proteome":null,"gene":"topB","go_terms":[{"identifier":"GO:0003677","name":"DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003916","name":"DNA topoisomerase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006265","name":"DNA topological change","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0003917","name":"DNA topoisomerase type I (single strand cut, ATP-independent) activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"50b5241a90332a8fc9fc2d4a42095c76d99ebd7a","counters":{"domain_architectures":15769,"entries":32,"isoforms":0,"proteomes":0,"sets":3,"structures":0,"taxa":1,"dbEntries":{"smart":3,"profile":2,"cdd":2,"ssf":1,"cathgene3d":4,"hamap":1,"ncbifam":2,"panther":1,"pfam":2,"prosite":1,"prints":1,"interpro":12},"proteome":0,"taxonomy":1,"similar_proteins":15769}}}